A7A2G1 · A7A2G1_YEAS7
- ProteinConserved protein
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1346 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Integrase (IN) targets the VLP to the nucleus, where a subparticle preintegration complex (PIC) containing at least integrase and the newly synthesized dsDNA copy of the retrotransposon must transit the nuclear membrane. Once in the nucleus, integrase performs the integration of the dsDNA into the host genome.
Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that catalyzes the conversion of the retro-elements RNA genome into dsDNA within the VLP. The enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA primers. The conversion leads to a linear dsDNA copy of the retrotransposon that includes long terminal repeats (LTRs) at both ends.
The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.
Catalytic activity
- DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
- DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | DNA-directed DNA polymerase activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA-directed DNA polymerase activity | |
Molecular Function | RNA-DNA hybrid ribonuclease activity | |
Biological Process | DNA integration | |
Biological Process | transposition |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Submitted names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionA7A2G1
Proteomes
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-16 | Polar residues | |||
Region | 1-24 | Disordered | |||
Domain | 232-407 | Integrase catalytic | |||
Region | 581-614 | Disordered | |||
Region | 635-711 | Disordered | |||
Compositional bias | 636-684 | Polar residues | |||
Region | 722-741 | Disordered | |||
Compositional bias | 746-763 | Polar residues | |||
Region | 746-781 | Disordered | |||
Compositional bias | 764-781 | Basic and acidic residues | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,346
- Mass (Da)153,884
- Last updated2007-09-11 v1
- Checksum76617AA72BE02296
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 1-16 | Polar residues | |||
Compositional bias | 636-684 | Polar residues | |||
Compositional bias | 746-763 | Polar residues | |||
Compositional bias | 764-781 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAFW02000252 EMBL· GenBank· DDBJ | EDN59031.1 EMBL· GenBank· DDBJ | Genomic DNA |