A7A2A4 · A7A2A4_YEAS7
- ProteinIntegrase catalytic domain-containing protein
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids604 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Integrase (IN) targets the VLP to the nucleus, where a subparticle preintegration complex (PIC) containing at least integrase and the newly synthesized dsDNA copy of the retrotransposon must transit the nuclear membrane. Once in the nucleus, integrase performs the integration of the dsDNA into the host genome.
Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that catalyzes the conversion of the retro-elements RNA genome into dsDNA within the VLP. The enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA primers. The conversion leads to a linear dsDNA copy of the retrotransposon that includes long terminal repeats (LTRs) at both ends.
Catalytic activity
- DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
- DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | RNA binding | |
Molecular Function | RNA-DNA hybrid ribonuclease activity | |
Biological Process | DNA integration | |
Biological Process | transposition |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameIntegrase catalytic domain-containing protein
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionA7A2A4
Proteomes
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 60-235 | Integrase catalytic | ||||
Sequence: NSYEPFQYLHTDIFGPVHNLPKSAPSYFISFTDETTKFRWVYPLHDRREDSILDVFTTILAFIKNQFQASVLVIQMDRGSEYTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRTLLDDCRTQLQCSGLPNHLWFSAIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLP | ||||||
Region | 356-604 | Disordered | ||||
Sequence: SKAVSPTDSTPPSTHTEDSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQISDIESTGSGGMHKLNVPLLAPMSQSETHESSHASKSKDFRHSDSYSDNETNHTNAPISSTGGTNNKTVPQTSEQETEKRIIHRSPSIDTSSSESNSLHHVVPIKTSDTCPKENTEESIIADLPLPDLPPEPPTELSDSFKELPPINSRQTNSSLGGIGDSNAYTTINSKKRSLEDNETEIKVSRDTWNTKNMRSL | ||||||
Compositional bias | 369-383 | Basic and acidic residues | ||||
Sequence: THTEDSKRVSKTNIR | ||||||
Compositional bias | 393-417 | Polar residues | ||||
Sequence: SESNILPSKKRSSTPQISDIESTGS | ||||||
Compositional bias | 439-456 | Basic and acidic residues | ||||
Sequence: SSHASKSKDFRHSDSYSD | ||||||
Compositional bias | 457-480 | Polar residues | ||||
Sequence: NETNHTNAPISSTGGTNNKTVPQT | ||||||
Compositional bias | 494-511 | Polar residues | ||||
Sequence: PSIDTSSSESNSLHHVVP | ||||||
Compositional bias | 549-578 | Polar residues | ||||
Sequence: KELPPINSRQTNSSLGGIGDSNAYTTINSK | ||||||
Compositional bias | 579-595 | Basic and acidic residues | ||||
Sequence: KRSLEDNETEIKVSRDT |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusFragment
- Length604
- Mass (Da)67,775
- Last updated2007-09-11 v1
- Checksum5FE487318DDCD59D
Features
Showing features for compositional bias, non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 369-383 | Basic and acidic residues | ||||
Sequence: THTEDSKRVSKTNIR | ||||||
Compositional bias | 393-417 | Polar residues | ||||
Sequence: SESNILPSKKRSSTPQISDIESTGS | ||||||
Compositional bias | 439-456 | Basic and acidic residues | ||||
Sequence: SSHASKSKDFRHSDSYSD | ||||||
Compositional bias | 457-480 | Polar residues | ||||
Sequence: NETNHTNAPISSTGGTNNKTVPQT | ||||||
Compositional bias | 494-511 | Polar residues | ||||
Sequence: PSIDTSSSESNSLHHVVP | ||||||
Compositional bias | 549-578 | Polar residues | ||||
Sequence: KELPPINSRQTNSSLGGIGDSNAYTTINSK | ||||||
Compositional bias | 579-595 | Basic and acidic residues | ||||
Sequence: KRSLEDNETEIKVSRDT | ||||||
Non-terminal residue | 604 | |||||
Sequence: L |
Keywords
- Technical term