A6W3C7 · TRMA_MARMS
- ProteintRNA/tmRNA (uracil-C(5))-methyltransferase
- GenetrmA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids366 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Dual-specificity methyltransferase that catalyzes the formation of 5-methyluridine at position 54 (m5U54) in all tRNAs, and that of position 341 (m5U341) in tmRNA (transfer-mRNA).
Catalytic activity
- S-adenosyl-L-methionine + uridine54 in tRNA = 5-methyluridine54 in tRNA + H+ + S-adenosyl-L-homocysteine
- S-adenosyl-L-methionine + uridine341 in tmRNA = 5-methyluridine341 in tmRNA + H+ + S-adenosyl-L-homocysteine
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 187 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 215 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 220 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 236 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 297 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 322 | Nucleophile | ||||
Sequence: C | ||||||
Active site | 356 | Proton acceptor | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | rRNA binding | |
Molecular Function | tRNA (uracil(54)-C5)-methyltransferase activity, S-adenosyl methionine-dependent | |
Molecular Function | tRNA binding | |
Biological Process | tRNA methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA/tmRNA (uracil-C(5))-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Oceanospirillaceae > Marinomonas
Accessions
- Primary accessionA6W3C7
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000084037 | 1-366 | tRNA/tmRNA (uracil-C5)-methyltransferase | |||
Sequence: MRPDQIQPEMYEKQLQDKQAELAKLMATLSLPEMEVFASQPTHYRMRAEFRIWHDGDDLYYAMFDSADPRTPIRTDQFLAASRLINELMPKLLDAVRDVPVLRYKLFQVDFLTTTTGEALISLLYHKPIDAEWNAAVQQLNNAFPACHFIGRSRKKKQIITRDFVMETLTVNGQKFHYQQVENSFTQPNAGISEKMLEWALDVTKEASGDLLEMYCGNGNFSIPLARRFDRVVATEISKVSVNSAQLNIAINGMHNVQVVKMASEDVSAALNGDVELPKSLVQAGVSELTPSVVLVDPPRAGLDDATVELIRKIDSILYISCNPETLKANLEALSNTHEVVRYAMFDQFPYTHHVETGVFLRRKAS |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length366
- Mass (Da)41,398
- Last updated2007-08-21 v1
- ChecksumFBA44BD478D16207
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000749 EMBL· GenBank· DDBJ | ABR73206.1 EMBL· GenBank· DDBJ | Genomic DNA |