A6W2I3 · PANB_MARMS
- Protein3-methyl-2-oxobutanoate hydroxymethyltransferase
- GenepanB
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids275 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.
Catalytic activity
- 3-methyl-2-oxobutanoate + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 55 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 55-56 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | |||
Binding site | 94 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | |||
Binding site | 94 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 122 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | |||
Binding site | 124 | Mg2+ (UniProtKB | ChEBI) | |||
Active site | 191 | Proton acceptor | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 3-methyl-2-oxobutanoate hydroxymethyltransferase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | pantothenate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-methyl-2-oxobutanoate hydroxymethyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Oceanospirillaceae > Marinomonas
Accessions
- Primary accessionA6W2I3
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000096982 | 1-275 | 3-methyl-2-oxobutanoate hydroxymethyltransferase | ||
Interaction
Structure
Sequence
- Sequence statusComplete
- Length275
- Mass (Da)29,642
- Last updated2007-08-21 v1
- ChecksumED111BE38D315D3E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000749 EMBL· GenBank· DDBJ | ABR72912.1 EMBL· GenBank· DDBJ | Genomic DNA |