A6W0S5 · MNMC_MARMS
- ProteintRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
- GenemnmC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids674 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.
Catalytic activity
- 5-aminomethyl-2-thiouridine34 in tRNA + S-adenosyl-L-methionine = 5-methylaminomethyl-2-thiouridine34 in tRNA + H+ + S-adenosyl-L-homocysteine
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | oxidoreductase activity, acting on the CH-NH group of donors | |
Molecular Function | tRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity | |
Biological Process | methylation | |
Biological Process | tRNA wobble base modification |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nametRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
- Short namestRNA mnm(5)s(2)U biosynthesis bifunctional protein
Including 2 domains:
- Recommended nametRNA (mnm(5)s(2)U34)-methyltransferase
- EC number
- Recommended nameFAD-dependent cmnm(5)s(2)U34 oxidoreductase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Oceanospirillales > Oceanospirillaceae > Marinomonas
Accessions
- Primary accessionA6W0S5
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000348002 | 1-674 | tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC | |||
Sequence: MLTSYQLESPALSWGEDGAPHSNQFDDVYFDKESGLEETRHVFLKNNQLSGRWASLQKNAFVIAETGFGTGLNFLCAWQAFLTEAASDKQLHFISVEKYPMTKSMLTDALKMWPSISHLSQQLIDAYPEVCHGLHRIELEQGRIQLTLWFGEAEDGFAALDADVDAWFLDGFAPSKNPEMWSDNLFKHIHRLSHQGTTFSTFTAAGIVRRGLKNVGFDVRKVKGFGQKREMTVGELNSSTAPLSARMSQGQAWFNLRQDKTSEITKVLVVGAGLAGANTAYALAKQGIKVEVWEQGNCIAGGASGNPQGMLYPKLASQDTPVNRFYLSAYLHATRLYSSLDRHKQFWDACGLIQIPKNDKEAVRFQKLLDEKLYPEAILQASKDREGCLFLPLSGWVVLTQLCETLLSHENIHVCLNTKLESLSPIELDDKTFGWQARSKYQQAPLNDRQACFSHVVLCTANDTKALGVTPKTPDYPIRGQVSFMDIEKAKAACQTIGESADKIDFDKILCEFGYVSPSINGLLHFGSTYDLKDLDDRVREEGHKRNLAILESLLLLPKETFNSEDCGGRVSFRCAVPDYTPIVGPVQSEDVYQQAYSTLTKNAKWQSNEIAEPIKQLYINIGHGSRGLISTPLSGSYIASLITGTPSPLEQKVSHKLHPSRFIIRDLKRSQIL |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-237 | tRNA (mnm5s2U34)-methyltransferase | ||||
Sequence: MLTSYQLESPALSWGEDGAPHSNQFDDVYFDKESGLEETRHVFLKNNQLSGRWASLQKNAFVIAETGFGTGLNFLCAWQAFLTEAASDKQLHFISVEKYPMTKSMLTDALKMWPSISHLSQQLIDAYPEVCHGLHRIELEQGRIQLTLWFGEAEDGFAALDADVDAWFLDGFAPSKNPEMWSDNLFKHIHRLSHQGTTFSTFTAAGIVRRGLKNVGFDVRKVKGFGQKREMTVGELN | ||||||
Region | 270-674 | FAD-dependent cmnm5s2U34 oxidoreductase | ||||
Sequence: VGAGLAGANTAYALAKQGIKVEVWEQGNCIAGGASGNPQGMLYPKLASQDTPVNRFYLSAYLHATRLYSSLDRHKQFWDACGLIQIPKNDKEAVRFQKLLDEKLYPEAILQASKDREGCLFLPLSGWVVLTQLCETLLSHENIHVCLNTKLESLSPIELDDKTFGWQARSKYQQAPLNDRQACFSHVVLCTANDTKALGVTPKTPDYPIRGQVSFMDIEKAKAACQTIGESADKIDFDKILCEFGYVSPSINGLLHFGSTYDLKDLDDRVREEGHKRNLAILESLLLLPKETFNSEDCGGRVSFRCAVPDYTPIVGPVQSEDVYQQAYSTLTKNAKWQSNEIAEPIKQLYINIGHGSRGLISTPLSGSYIASLITGTPSPLEQKVSHKLHPSRFIIRDLKRSQIL |
Sequence similarities
In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.
In the C-terminal section; belongs to the DAO family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length674
- Mass (Da)75,186
- Last updated2007-08-21 v1
- ChecksumB47DB9DF3234ABAA
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000749 EMBL· GenBank· DDBJ | ABR72304.1 EMBL· GenBank· DDBJ | Genomic DNA |