A6UE14 · RLMN_SINMW

Function

function

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site126Proton acceptor
Binding site146[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site150[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site153[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site210-211S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site242S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site264-266S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site341S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site384S-methylcysteine intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular FunctionrRNA (adenine(2503)-C2-)-methyltransferase activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA (adenine(37)-C2)-methyltransferase activity
Molecular FunctiontRNA binding
Biological ProcessrRNA base methylation
Biological ProcesstRNA methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dual-specificity RNA methyltransferase RlmN
  • EC number
  • Alternative names
    • 23S rRNA (adenine(2503)-C(2))-methyltransferase
    • 23S rRNA m2A2503 methyltransferase
    • Ribosomal RNA large subunit methyltransferase N
    • tRNA (adenine(37)-C(2))-methyltransferase
    • tRNA m2A37 methyltransferase

Gene names

    • Name
      rlmN
    • Ordered locus names
      Smed_3068

Organism names

Accessions

  • Primary accession
    A6UE14

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00003504171-413Dual-specificity RNA methyltransferase RlmN
Disulfide bond139↔384(transient)

Keywords

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain132-381Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. RlmN family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    413
  • Mass (Da)
    46,251
  • Last updated
    2007-08-21 v1
  • Checksum
    6561E70B422ED884
MSMAATETLNRTDIAKAPQVRLAAQPEKPSLIGLLREDMAKLLVEKGVPERQVKMRVSQVWHWLYVRGVSDFNEMSNVSKDMREMLSAHFTIARPEIVEEQVSGDGTRKWLLRFPPRGAGRPVEIETVYIPEEGRGTLCISSQVGCTLTCSFCHTGTQKLVRNLTAEEILAQLLLARDRLGDFPDRDTPQGAIVPAEGRKITNVVMMGMGEPLYNFENVKTALLIASDGDGLSLSKRRITLSTSGIVPEIYRTGEEIGVMLAISLHAVRDDLRDMLVPINKKYPLKELMEACRAYPGLSNARRITFEYVMLKDVNDSLEDAKELVKLLKGIPAKINLIPFNPWPGTNYQCSDWEQIEKFADFINQAGYASPIRTPRGRDILAACGQLKSDSERMRKVDRLAFEAMMIANHGED

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000738
EMBL· GenBank· DDBJ
ABR61894.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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