A6T5F6 · THII_KLEP7

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site183-184ATP (UniProtKB | ChEBI)
Binding site265ATP (UniProtKB | ChEBI)
Binding site287ATP (UniProtKB | ChEBI)
Binding site296ATP (UniProtKB | ChEBI)
Active site456Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA thio-modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      KPN_00375
    • Ordered locus names
      KPN78578_03660

Organism names

Accessions

  • Primary accession
    A6T5F6

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_10000742311-482tRNA sulfurtransferase
Disulfide bond344↔456Redox-active

Keywords

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain61-165THUMP
Domain404-482Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    482
  • Mass (Da)
    54,717
  • Last updated
    2007-08-21 v1
  • Checksum
    6E261ED11F8CBAEE
MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKNYDETLAVVRHWDHIEVRAKDENQRPAIRDALTRIPGIHHILEVEDVPFTSLHDIFEQTLPLWREALEGKTFCVRVKRRGKHEFTSIEVERYVGGGLNQHIETARVKLTDPDVTVNLEIENDRLLLVKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEIGVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKVDDGQMGVVLKRMMVRAASKVAERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISHDKEHIIDLAREIGTEDFARTMPEYCGVISKSPTVKAVKAKIEAEEEHFDFSILDKVVEEASNIDIREIAQQTEETVVEVETVTGFGANDAILDIRSIDEQEDKPLKVEGVEVVSLPFYKLSTKFGDLDQSKTWLLWCERGVMSRLQALYLREQGFSNVKVYRP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000647
EMBL· GenBank· DDBJ
ABR75827.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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