Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

A6QS12 · PFF1_AJECN

Function

function

May be involved in vacuolar sorting and osmoregulation.

Caution

Although related to the peptidase M28 family, it lacks some conserved zinc-binding and active site residues and therefore has probably lost hydrolase activity.

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per subunit.

Features

Showing features for binding site, active site.

1850100200300400500600700800
Type
IDPosition(s)Description
Binding site175Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site187Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site187Zn2+ 2 (UniProtKB | ChEBI); catalytic
Active site221Proton acceptor
Binding site222Zn2+ 2 (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Cellular Componentvacuolar membrane
Molecular Functionmetal ion binding
Molecular Functionmetalloexopeptidase activity
Biological Processproteolysis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Vacuolar membrane protease
  • EC number
  • Alternative names
    • FXNA-related family protease 1

Gene names

    • ORF names
      HCAG_00168

Organism names

Accessions

  • Primary accession
    A6QS12

Proteomes

Organism-specific databases

Subcellular Location

Vacuole membrane
; Multi-pass membrane protein

Features

Showing features for topological domain, transmembrane.

Type
IDPosition(s)Description
Topological domain1-20Cytoplasmic
Transmembrane21-41Helical; Name=1
Topological domain42-282Vacuolar
Transmembrane283-303Helical; Name=2
Topological domain304-308Cytoplasmic
Transmembrane309-329Helical; Name=3
Topological domain330-363Vacuolar
Transmembrane364-384Helical; Name=4
Topological domain385-393Cytoplasmic
Transmembrane394-414Helical; Name=5
Topological domain415-425Vacuolar
Transmembrane426-446Helical; Name=6
Topological domain447-529Cytoplasmic
Transmembrane530-550Helical; Name=7
Topological domain551-563Vacuolar
Transmembrane564-584Helical; Name=8
Topological domain585-590Cytoplasmic
Transmembrane591-611Helical; Name=9
Topological domain612-850Vacuolar

Keywords

PTM/Processing

Features

Showing features for chain, glycosylation.

Type
IDPosition(s)Description
ChainPRO_00004116911-850Vacuolar membrane protease
Glycosylation53N-linked (GlcNAc...) asparagine
Glycosylation116N-linked (GlcNAc...) asparagine
Glycosylation119N-linked (GlcNAc...) asparagine
Glycosylation630N-linked (GlcNAc...) asparagine
Glycosylation658N-linked (GlcNAc...) asparagine
Glycosylation702N-linked (GlcNAc...) asparagine

Keywords

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the peptidase M28 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    850
  • Mass (Da)
    94,074
  • Last updated
    2007-08-21 v1
  • MD5 Checksum
    8DC8CEFA0414AF9B53952B529DF3F6C3
MASSRAQWFNPIAFTPWPVTCITTIVYLALLIPILVINLVVPSAPETNPKGVNLTEAWRDLQHLTGGFHPYNSRRNDEVHEWLLSRINSIIRPTVEAGQPSSANDNLPEVFVFDDNRSNLTYSNGGVGKTSIVGVYFESTNIIVYIRGSEDGLENWWEHSNGKPKGKGGVLVNAHYDSVSTGYGATDDGIGVVSLLQLLRYFTTPGNNPRKGLVLLFNNGEEDYLNGAHPPEADTTQAQDDTRHTNIDSLWHMLSASIGTTEGLVSYTGMDFDGKSKDQNKVNSGTGTLGVWFDMFGTAFAVFRLHTLFAISVALLVIAPLVIFVTSVILSKTDRMYLFSMSKSLEGTGDQVSLRGLRGFSRTPIILVIATTIPICLAYLLEKVNPYIVHSSQFSVWSMMFSAWIFLAWFLACAADFFRPSALHRAYSYTWIFIATWIMLVINTVYANQKGIAAGPSTAEFPGAAGEDTDPTESTSLLRGQRTTFANYRSSRPGGAAETDEREDINKGGTFEHEQSWSWTLPRWTWVLQLLLLAPIVLILVGQLALFLTASMCQVGSDGVSTFVVYLACSVFTTLLCIPLFPLIHRFTYHIPTFLFLVFIGTLIYNLVAFPFSPANRLKTFFIQEVDLDNGSNTVSLTGIQPYLTDAINSIPSAAGQNITCDKTTPFGKLERCSWSGLSPNVLGQGRERDNEIDPDKWITYNITKTVGKNKARIEISGRNTRACKLKFDRAVANFQVSGSAVDHRMPPTSRQGVSEIRLWSRTWENTWVVDINWHESADKSDDDDDDHDDENHDAPQNILSGKAICMWSDGNQPGVIPALDEVRLYAPSWIAISKAADGLVEASHSFTIQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CH476655
EMBL· GenBank· DDBJ
EDN02304.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help