A6HNQ5 · A6HNQ5_RAT
- ProteinMethylthioribulose-1-phosphate dehydratase
- GeneApip
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids241 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.
Catalytic activity
- 5-(methylsulfanyl)ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O
Cofactor
Note: Binds 1 zinc ion per subunit.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 2/6.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 96 | substrate | |||
Binding site | 114 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 116 | Zn2+ (UniProtKB | ChEBI) | |||
Active site | 138 | Proton donor/acceptor | |||
Binding site | 194 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | cytoskeleton | |
Cellular Component | membrane | |
Molecular Function | identical protein binding | |
Molecular Function | methylthioribulose 1-phosphate dehydratase activity | |
Molecular Function | zinc ion binding | |
Biological Process | apoptotic process | |
Biological Process | L-methionine salvage from methylthioadenosine | |
Biological Process | L-methionine salvage from S-adenosylmethionine | |
Biological Process | negative regulation of apoptotic process | |
Biological Process | protein homotetramerization | |
Biological Process | pyroptotic inflammatory response | |
Biological Process | regulation of ERK1 and ERK2 cascade |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethylthioribulose-1-phosphate dehydratase
- EC number
- Short namesMTRu-1-P dehydratase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionA6HNQ5
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endomembrane system ; Peripheral membrane protein
Keywords
- Cellular component
Interaction
Subunit
Homotetramer. Interacts with APAF1. May interact with CASP1.
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 25-221 | Class II aldolase/adducin N-terminal | |||
Sequence similarities
Belongs to the aldolase class II family. Adducin subfamily.
Belongs to the aldolase class II family. MtnB subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length241
- Mass (Da)27,053
- Last updated2023-06-28 v1
- MD5 Checksum9019C8F6E4C4D80F7CC1DC16C9AD28D3
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CH473949 EMBL· GenBank· DDBJ | EDL79656.1 EMBL· GenBank· DDBJ | Genomic DNA |