A5WDM3 · A5WDM3_PSYWF
- ProteinUDP-2,3-diacylglucosamine hydrolase
- GenelpxH
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids296 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Hydrolyzes the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP by catalyzing the attack of water at the alpha-P atom. Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
Catalytic activity
- UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosamine + H2O = 2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-alpha-D-glucosaminyl 1-phosphate + UMP + 2 H+
Cofactor
Note: Binds 2 Mn2+ ions per subunit in a binuclear metal center.
Pathway
Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 4/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 29 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 31 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 64 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 64 | Mn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 111 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 111-112 | substrate | |||
Binding site | 146 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 154 | substrate | |||
Binding site | 192 | substrate | |||
Binding site | 196 | substrate | |||
Binding site | 199 | substrate | |||
Binding site | 225 | Mn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 225 | substrate | |||
Binding site | 227 | Mn2+ 1 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | extrinsic component of plasma membrane | |
Molecular Function | manganese ion binding | |
Molecular Function | UDP-2,3-diacylglucosamine hydrolase activity | |
Biological Process | lipid A biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameUDP-2,3-diacylglucosamine hydrolase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Moraxellales > Moraxellaceae > Psychrobacter
Accessions
- Primary accessionA5WDM3
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Peripheral membrane protein
Keywords
- Cellular component
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length296
- Mass (Da)33,948
- Last updated2007-07-10 v1
- Checksum0253A9D647543D7E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000713 EMBL· GenBank· DDBJ | ABQ93764.1 EMBL· GenBank· DDBJ | Genomic DNA |