A5VHK2 · ADDA_LIMRD

Function

function

The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site25-32ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular ComponentDNA helicase complex
Molecular Function3'-5' DNA helicase activity
Molecular Function3'-5' exonuclease activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functiondouble-stranded DNA binding
Molecular Functionisomerase activity
Biological Processdouble-strand break repair via homologous recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent helicase/nuclease subunit A
  • EC number
  • Alternative names
    • ATP-dependent helicase/nuclease AddA
    • DNA 3'-5' helicase AddA

Gene names

    • Name
      addA
    • Ordered locus names
      Lreu_0050

Organism names

Accessions

  • Primary accession
    A5VHK2

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003792861-1392ATP-dependent helicase/nuclease subunit A

Interaction

Subunit

Heterodimer of AddA and AddB/RexB.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-595UvrD-like helicase ATP-binding
Domain623-929UvrD-like helicase C-terminal

Sequence similarities

Belongs to the helicase family. AddA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,392
  • Mass (Da)
    159,710
  • Last updated
    2007-07-10 v1
  • Checksum
    FF9599F8233A0394
MAGFKPTPAQSKAINDRGENILVSASAGSGKTAVLVNRTIELIKEGQSIDRMLLVTFTDAAAKNMRDKIRAALQKIVQDSANPKDLRDRMSNQINRLAAADISTIHAFCLKLIKRYYYLIDLDPQFRLLTDETERLLLQEDVWHEVSEELYRNAEEKVSGKASFSELVLNFSSDRDDQGLDDLILRLYEIANAQPDPEKWLQKLPDNYDLGSGSLLESNFYQQQLKPLVIEKLNQFIQDYRELVTRASDNGLDQAAEVIKSDEELMHQLLSSLGGITVSDVCQMMAQQKFGSFRGRPAADDPRIDVFKDIQKQRNQLKKQWEQMVSTYLGKQEAQEPIAKEELLTELTTFSDQFTDLLSKATESQLDAKTVDSLQKDQQMMQELLDLLQPPTWNTIRDLFANAKFARMGGKPKDDELAEEVYKSLGSARTGIKKQFDQLVDRFFNYREDQFRLISTHAQELLRELSAVTINFRRRYQQTKLNRHVLEFSDLEHYAYAILTPPDDQPNWQTLVKDLQNHYQEIMIDEYQDTNRLQESILMKLTSPERKNLFMVGDVKQSIYRFREADPTLFLGKYQNYRQGSDGEAIVLGENFRSMTNVTSFTNILFEQLMDREVGEIDYDEDAHLKYAATYYEENQDNKVHPTEVLLYDANALDPEKEDVEHEDDKLAGEFRMIGMRIKQMVENQELIFHPEDGQMHPIQYGDIVLLERTKAINNSLMEEFNKLNIPLTVHDVESYFQATEVRVMMSLLKIIDNPQQDIPLVAVLRSPIVGLTNQELAFIRLQNRSVDYYAALQTFMSNYQRKALRHQSLLTSEQVNALYEKADHFLGLLRVFRQTAQQQTLVDLIWQIYDQTGYLDYVGAMPGGHQRQANLHALYQRAHSYEQSSFKGLYQFIRFIEKMQEHDKDLGVAPTQLTANTVNVMTIHGSKGLQFPVVFLIDATHGFNKGAARENAVVDAVAGVGIRYMDDQRVIYDTPQRQAVIEEIQRGERAEDLRVLYVALTRAEQRLVITGSFNEEMRTQSLAGSWQRWQKAYQSKNLLIGPQPRITANSFMDWVGLALARYPEFNAQQLSRGNVTLEESTLADTKVTGLAADPHFTAKTYTALDVSDGLAKIGQNASANVTEKNNTVATDASEQKIEQILRYRYPHLVATKTTAYQSVTDVKRVFEDPDTRDMARWDYDQQQKVKTQGIYLNNNFDVPAFIQQTTHEPVATEIGTATHLVFQKLPLDEGLINVEFVDQEIQKLVGEKLINPVVAARINREGIVAFYQTAVGQKILKHPADYHREVPFSMIMNGHELFKGVNVSDDERILIHGIIDGYLRTDEGIILVDYKNDHLNKDYRDFDLARIKDRYRGQLELYKEALNLMEGIPVVQMGLYLLELGEFVLFTKEGD

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000705
EMBL· GenBank· DDBJ
ABQ82326.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp