A5VHK2 · ADDA_LIMRD
- ProteinATP-dependent helicase/nuclease subunit A
- GeneaddA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1392 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddA nuclease domain is required for chi fragment generation; this subunit has the helicase and 3' -> 5' nuclease activities.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Cofactor
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | DNA helicase complex | |
Molecular Function | 3'-5' DNA helicase activity | |
Molecular Function | 3'-5' exonuclease activity | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | double-stranded DNA binding | |
Molecular Function | isomerase activity | |
Biological Process | double-strand break repair via homologous recombination |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent helicase/nuclease subunit A
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Limosilactobacillus
Accessions
- Primary accessionA5VHK2
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000379286 | 1-1392 | ATP-dependent helicase/nuclease subunit A | |||
Sequence: MAGFKPTPAQSKAINDRGENILVSASAGSGKTAVLVNRTIELIKEGQSIDRMLLVTFTDAAAKNMRDKIRAALQKIVQDSANPKDLRDRMSNQINRLAAADISTIHAFCLKLIKRYYYLIDLDPQFRLLTDETERLLLQEDVWHEVSEELYRNAEEKVSGKASFSELVLNFSSDRDDQGLDDLILRLYEIANAQPDPEKWLQKLPDNYDLGSGSLLESNFYQQQLKPLVIEKLNQFIQDYRELVTRASDNGLDQAAEVIKSDEELMHQLLSSLGGITVSDVCQMMAQQKFGSFRGRPAADDPRIDVFKDIQKQRNQLKKQWEQMVSTYLGKQEAQEPIAKEELLTELTTFSDQFTDLLSKATESQLDAKTVDSLQKDQQMMQELLDLLQPPTWNTIRDLFANAKFARMGGKPKDDELAEEVYKSLGSARTGIKKQFDQLVDRFFNYREDQFRLISTHAQELLRELSAVTINFRRRYQQTKLNRHVLEFSDLEHYAYAILTPPDDQPNWQTLVKDLQNHYQEIMIDEYQDTNRLQESILMKLTSPERKNLFMVGDVKQSIYRFREADPTLFLGKYQNYRQGSDGEAIVLGENFRSMTNVTSFTNILFEQLMDREVGEIDYDEDAHLKYAATYYEENQDNKVHPTEVLLYDANALDPEKEDVEHEDDKLAGEFRMIGMRIKQMVENQELIFHPEDGQMHPIQYGDIVLLERTKAINNSLMEEFNKLNIPLTVHDVESYFQATEVRVMMSLLKIIDNPQQDIPLVAVLRSPIVGLTNQELAFIRLQNRSVDYYAALQTFMSNYQRKALRHQSLLTSEQVNALYEKADHFLGLLRVFRQTAQQQTLVDLIWQIYDQTGYLDYVGAMPGGHQRQANLHALYQRAHSYEQSSFKGLYQFIRFIEKMQEHDKDLGVAPTQLTANTVNVMTIHGSKGLQFPVVFLIDATHGFNKGAARENAVVDAVAGVGIRYMDDQRVIYDTPQRQAVIEEIQRGERAEDLRVLYVALTRAEQRLVITGSFNEEMRTQSLAGSWQRWQKAYQSKNLLIGPQPRITANSFMDWVGLALARYPEFNAQQLSRGNVTLEESTLADTKVTGLAADPHFTAKTYTALDVSDGLAKIGQNASANVTEKNNTVATDASEQKIEQILRYRYPHLVATKTTAYQSVTDVKRVFEDPDTRDMARWDYDQQQKVKTQGIYLNNNFDVPAFIQQTTHEPVATEIGTATHLVFQKLPLDEGLINVEFVDQEIQKLVGEKLINPVVAARINREGIVAFYQTAVGQKILKHPADYHREVPFSMIMNGHELFKGVNVSDDERILIHGIIDGYLRTDEGIILVDYKNDHLNKDYRDFDLARIKDRYRGQLELYKEALNLMEGIPVVQMGLYLLELGEFVLFTKEGD |
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-595 | UvrD-like helicase ATP-binding | ||||
Sequence: FKPTPAQSKAINDRGENILVSASAGSGKTAVLVNRTIELIKEGQSIDRMLLVTFTDAAAKNMRDKIRAALQKIVQDSANPKDLRDRMSNQINRLAAADISTIHAFCLKLIKRYYYLIDLDPQFRLLTDETERLLLQEDVWHEVSEELYRNAEEKVSGKASFSELVLNFSSDRDDQGLDDLILRLYEIANAQPDPEKWLQKLPDNYDLGSGSLLESNFYQQQLKPLVIEKLNQFIQDYRELVTRASDNGLDQAAEVIKSDEELMHQLLSSLGGITVSDVCQMMAQQKFGSFRGRPAADDPRIDVFKDIQKQRNQLKKQWEQMVSTYLGKQEAQEPIAKEELLTELTTFSDQFTDLLSKATESQLDAKTVDSLQKDQQMMQELLDLLQPPTWNTIRDLFANAKFARMGGKPKDDELAEEVYKSLGSARTGIKKQFDQLVDRFFNYREDQFRLISTHAQELLRELSAVTINFRRRYQQTKLNRHVLEFSDLEHYAYAILTPPDDQPNWQTLVKDLQNHYQEIMIDEYQDTNRLQESILMKLTSPERKNLFMVGDVKQSIYRFREADPTLFLGKYQNYRQGSDGEAIVLGENFRSM | ||||||
Domain | 623-929 | UvrD-like helicase C-terminal | ||||
Sequence: AHLKYAATYYEENQDNKVHPTEVLLYDANALDPEKEDVEHEDDKLAGEFRMIGMRIKQMVENQELIFHPEDGQMHPIQYGDIVLLERTKAINNSLMEEFNKLNIPLTVHDVESYFQATEVRVMMSLLKIIDNPQQDIPLVAVLRSPIVGLTNQELAFIRLQNRSVDYYAALQTFMSNYQRKALRHQSLLTSEQVNALYEKADHFLGLLRVFRQTAQQQTLVDLIWQIYDQTGYLDYVGAMPGGHQRQANLHALYQRAHSYEQSSFKGLYQFIRFIEKMQEHDKDLGVAPTQLTANTVNVMTIHGSKG |
Sequence similarities
Belongs to the helicase family. AddA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,392
- Mass (Da)159,710
- Last updated2007-07-10 v1
- ChecksumFF9599F8233A0394
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000705 EMBL· GenBank· DDBJ | ABQ82326.1 EMBL· GenBank· DDBJ | Genomic DNA |