A5UFJ9 · LIPB_HAEIG
- ProteinOctanoyltransferase
- GenelipB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids212 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate.
Miscellaneous
In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.
Catalytic activity
- L-lysyl-[protein] + octanoyl-[ACP] = H+ + holo-[ACP] + N6-octanoyl-L-lysyl-[protein]
Pathway
Protein modification; protein lipoylation via endogenous pathway; protein N6-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 70-77 | substrate | ||||
Sequence: RGGQITYH | ||||||
Site | 135 | Lowers pKa of active site Cys | ||||
Sequence: K | ||||||
Binding site | 138-140 | substrate | ||||
Sequence: SLG | ||||||
Binding site | 151-153 | substrate | ||||
Sequence: GLA | ||||||
Active site | 169 | Acyl-thioester intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | lipoyl(octanoyl) transferase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameOctanoyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pasteurellales > Pasteurellaceae > Haemophilus
Accessions
- Primary accessionA5UFJ9
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000001105 | 1-212 | Octanoyltransferase | |||
Sequence: MNNSLIVRQLGLQDYQEIWHKMQDFTDTRNAETQDEIWLVQHYPVFTQGQAGKPEHLLQRSEIPVVQSDRGGQITYHAPGQQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIESYPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDLNPFHYINPCGYAGLEMCQLADFVNQDEADWDNVSAKLIKHFADLLGYNITTL |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 31-209 | BPL/LPL catalytic | ||||
Sequence: AETQDEIWLVQHYPVFTQGQAGKPEHLLQRSEIPVVQSDRGGQITYHAPGQQVMYVLIDIKRHKNLNVRQLVTALEQTVVKTLAEYGIESYPKPDAPGVYVDGKKICSLGLRIRRGCSFHGLALNINMDLNPFHYINPCGYAGLEMCQLADFVNQDEADWDNVSAKLIKHFADLLGYNI |
Sequence similarities
Belongs to the LipB family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length212
- Mass (Da)24,159
- Last updated2007-07-10 v1
- Checksum126F2476EA74743E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000672 EMBL· GenBank· DDBJ | ABQ99554.1 EMBL· GenBank· DDBJ | Genomic DNA |