A5U5B1 · EIS_MYCTA
- ProteinN-acetyltransferase Eis
- Geneeis
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids402 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Effector that is released into the host cell and affects host immune responses. Acts as an acetyltransferase that acetylates lysine residues of host proteins.
Probably acetylates endogenous DNA-binding protein HU homolog (HupB) in vivo; overexpression (in M.smegmatis) leads to nucleoid decompaction, possibly via acetylation of HupB (PubMed:26817737).
Also succinylates HupB (PubMed:34224344).
Also succinylates HupB (PubMed:34224344).
Catalytic activity
- acetyl-CoA + L-lysyl-[protein] = CoA + H+ + N6-acetyl-L-lysyl-[protein]
- L-lysyl-[protein] + succinyl-CoA = CoA + H+ + N6-succinyl-L-lysyl-[protein]
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.4 μM | acetylation of HupB | 8.0 | 28 | |||
1.05 μM | acetylation of HupB | 8.0 | 25 | |||
0.29 μM | succinylation of HupB | 8.0 | 25 |
kcat is 1.91 sec-1 for acetylation and 2.43 sec-1 for succinylation of HupB.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 85-87 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: VAV | ||||||
Binding site | 93-98 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: RRGLLR | ||||||
Binding site | 121-122 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: SE | ||||||
Active site | 126 | Proton donor | ||||
Sequence: Y | ||||||
Active site | 402 | Proton acceptor; via carboxylate | ||||
Sequence: F |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | bacterial extracellular vesicle | |
Cellular Component | host cell cytoplasmic vesicle | |
Cellular Component | host extracellular space | |
Molecular Function | peptide-lysine-N-acetyltransferase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetyltransferase Eis
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionA5U5B1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000458843 | 1-402 | N-acetyltransferase Eis | |||
Sequence: MTVTLCSPTEDDWPGMFLLAAASFTDFIGPESATAWRTLVPTDGAVVVRDGAGPGSEVVGMALYMDLRLTVPGEVVLPTAGLSFVAVAPTHRRRGLLRAMCAELHRRIADSGYPVAALHASEGGIYGRFGYGPATTLHELTVDRRFARFHADAPGGGLGGSSVRLVRPTEHRGEFEAIYERWRQQVPGGLLRPQVLWDELLAECKAAPGGDRESFALLHPDGYALYRVDRTDLKLARVSELRAVTADAHCALWRALIGLDSMERISIITHPQDPLPHLLTDTRLARTTWRQDGLWLRIMNVPAALEARGYAHEVGEFSTVLEVSDGGRFALKIGDGRARCTPTDAAAEIEMDRDVLGSLYLGAHRASTLAAANRLRTKDSQLLRRLDAAFASDVPVQTAFEF |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 3-154 | N-acetyltransferase | ||||
Sequence: VTLCSPTEDDWPGMFLLAAASFTDFIGPESATAWRTLVPTDGAVVVRDGAGPGSEVVGMALYMDLRLTVPGEVVLPTAGLSFVAVAPTHRRRGLLRAMCAELHRRIADSGYPVAALHASEGGIYGRFGYGPATTLHELTVDRRFARFHADAP |
Sequence similarities
Belongs to the acetyltransferase Eis family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length402
- Mass (Da)43,804
- Last updated2007-07-10 v1
- ChecksumEF06F75C00F05333
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000611 EMBL· GenBank· DDBJ | ABQ74211.1 EMBL· GenBank· DDBJ | Genomic DNA |