A5U3K4 · MASZ_MYCTA
- ProteinMalate synthase G
- GeneglcB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids741 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.
Catalytic activity
- acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H+
Cofactor
Pathway
Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 118 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 125-126 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: RF | ||||||
Binding site | 275 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 312 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 339 | Proton acceptor | ||||
Sequence: R | ||||||
Binding site | 339 | glyoxylate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 434 | glyoxylate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 434 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 459-462 | glyoxylate (UniProtKB | ChEBI) | ||||
Sequence: GFLD | ||||||
Binding site | 462 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 543 | acetyl-CoA (UniProtKB | ChEBI) | ||||
Sequence: P | ||||||
Active site | 633 | Proton donor | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cell surface | |
Cellular Component | cytoplasm | |
Molecular Function | malate synthase activity | |
Molecular Function | metal ion binding | |
Biological Process | glyoxylate cycle | |
Biological Process | tricarboxylic acid cycle |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMalate synthase G
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionA5U3K4
Proteomes
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Knockdown of the enzyme results in increased sensitivity to ethyl bromopyruvate and a decreased cell population growth on glycine carbon source.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000056910 | 1-741 | Malate synthase G | |||
Sequence: MTDRVSVGNLRIARVLYDFVNNEALPGTDIDPDSFWAGVDKVVADLTPQNQALLNARDELQAQIDKWHRRRVIEPIDMDAYRQFLTEIGYLLPEPDDFTITTSGVDAEITTTAGPQLVVPVLNARFALNAANARWGSLYDALYGTDVIPETDGAEKGPTYNKVRGDKVIAYARKFLDDSVPLSSGSFGDATGFTVQDGQLVVALPDKSTGLANPGQFAGYTGAAESPTSVLLINHGLHIEILIDPESQVGTTDRAGVKDVILESAITTIMDFEDSVAAVDAADKVLGYRNWLGLNKGDLAAAVDKDGTAFLRVLNRDRNYTAPGGGQFTLPGRSLMFVRNVGHLMTNDAIVDTDGSEVFEGIMDALFTGLIAIHGLKASDVNGPLINSRTGSIYIVKPKMHGPAEVAFTCELFSRVEDVLGLPQNTMKIGIMDEERRTTVNLKACIKAAADRVVFINTGFLDRTGDEIHTSMEAGPMVRKGTMKSQPWILAYEDHNVDAGLAAGFSGRAQVGKGMWTMTELMADMVETKIAQPRAGASTAWVPSPTAATLHALHYHQVDVAAVQQGLAGKRRATIEQLLTIPLAKELAWAPDEIREEVDNNCQSILGYVVRWVDQGVGCSKVPDIHDVALMEDRATLRISSQLLANWLRHGVITSADVRASLERMAPLVDRQNAGDVAYRPMAPNFDDSIAFLAAQELILSGAQQPNGYTEPILHRRRREFKARAAEKPAPSDRAGDDAAR | ||||||
Modified residue | 619 | Cysteine sulfenic acid (-SOH) | ||||
Sequence: C |
Keywords
- PTM
Expression
Induction
Repressed in hypoxic conditions.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 718-741 | Disordered | ||||
Sequence: RREFKARAAEKPAPSDRAGDDAAR |
Sequence similarities
Belongs to the malate synthase family. GlcB subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length741
- Mass (Da)80,403
- Last updated2007-07-10 v1
- ChecksumA92F54E0FE8B7C64
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000611 EMBL· GenBank· DDBJ | ABQ73604.1 EMBL· GenBank· DDBJ | Genomic DNA |