A5TY85 · PKNA_MYCTA

Function

function

Protein kinase that regulates many aspects of mycobacterial physiology. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins such as FtsZ and MurD. Shows a strong preference for Thr versus Ser as the phosphoacceptor.

Catalytic activity

Activity regulation

Activated by magnesium or manganese. Inhibited by vanadate.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site19-27ATP (UniProtKB | ChEBI)
Binding site42ATP (UniProtKB | ChEBI)
Active site141Proton acceptor

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine kinase activity
Biological Processregulation of primary metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Serine/threonine-protein kinase PknA
  • EC number

Gene names

    • Name
      pknA
    • Ordered locus names
      MRA_0017

Organism names

Accessions

  • Primary accession
    A5TY85

Proteomes

Subcellular Location

Cell membrane
; Single-pass membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-339Cytoplasmic
Transmembrane340-360Helical
Topological domain361-431Extracellular

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis42Lack of autophosphorylation.
Mutagenesis172Strong decrease in autophosphorylation.
Mutagenesis174Decrease in autophosphorylation.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004197421-431Serine/threonine-protein kinase PknA

Post-translational modification

Autophosphorylated.

Keywords

Interaction

Subunit

Interacts with FtsZ and MurD.

Structure

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain13-272Protein kinase
Region276-333Disordered
Compositional bias302-316Polar residues
Compositional bias366-394Polar residues
Region366-418Disordered
Compositional bias403-417Polar residues

Domain

The kinase domain and the juxtamembrane region constitute the catalytic core of PknA. Interaction between core and C-terminal domains is crucial for activity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    431
  • Mass (Da)
    45,597
  • Last updated
    2007-07-10 v1
  • Checksum
    582D183747F3C111
MSPRVGVTLSGRYRLQRLIATGGMGQVWEAVDNRLGRRVAVKVLKSEFSSDPEFIERFRAEARTTAMLNHPGIASVHDYGESQMNGEGRTAYLVMELVNGEPLNSVLKRTGRLSLRHALDMLEQTGRALQIAHAAGLVHRDVKPGNILITPTGQVKITDFGIAKAVDAAPVTQTGMVMGTAQYIAPEQALGHDASPASDVYSLGVVGYEAVSGKRPFAGDGALTVAMKHIKEPPPPLPPDLPPNVRELIEITLVKNPAMRYRSGGPFADAVAAVRAGRRPPRPSQTPPPGRAAPAAIPSGTTARVAANSAGRTAASRRSRPATGGHRPPRRTFSSGQRALLWAAGVLGALAIIIAVLLVIKAPGDNSPQQAPTPTVTTTGNPPASNTGGTDASPRLNWTERGETRHSGLQSWVVPPTPHSRASLARYEIAQ

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias302-316Polar residues
Compositional bias366-394Polar residues
Compositional bias403-417Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000611
EMBL· GenBank· DDBJ
ABQ71735.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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