A5IXT6 · TRMFO_MYCAP
- ProteinMethylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
- GenetrmFO
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids427 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.
Catalytic activity
- uridine54 in tRNA + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADH + H+ = 5-methyluridine54 in tRNA + (6S)-5,6,7,8-tetrahydrofolate + NAD+
RHEA-COMP:10193 CHEBI:65315 Position: 54+ CHEBI:15636 + CHEBI:57945 + CHEBI:15378 = RHEA-COMP:10167 CHEBI:74447 Position: 54+ CHEBI:57453 + CHEBI:57540
Cofactor
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | tRNA (uracil(54)-C5)-methyltransferase activity, 5,10-methylenetetrahydrofolate-dependent | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Mycoplasmatota > Mycoplasmoidales > Metamycoplasmataceae > Mycoplasmopsis
Accessions
- Primary accessionA5IXT6
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000346361 | 1-427 | Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO | |||
Sequence: MKRVRVIGAGISGSEACYQLLKRNYLVELFEVKSIKKNPIQTNDMFANLAYSDSFHSNEITSAKGLLKQEMRLLDSLIINAADYAKVANEPLLVDRYKFQEYITNYLRSHQNLKIIEKEYITIDDSVPTIIATGPLSSVNLENEIKNLVGESNFNLFDKVEPIVLKSSINLNYVQKSALDDKLFYCTLNKNEFEYLYNLIISAEIFVSPLPNEIKLLHDNGFRSIETVAKSSKNELKNLLQANEIRQTENTYATVLLREDDYNENFLRIVNFQTSIKIPWQNEIIKAVPALANALILRYGVMHKNDYINSANVLDDNFQLKSNPNIFFAGQLTGTDGYVEASASAIICAINVDRYLRNLPKAIPNNKTVIGSLCNYVLIANKEDFQPMEANWGLVESMNLVSYNNEGKKKLSDRAISEIKEFIKQNL |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the MnmG family. TrmFO subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length427
- Mass (Da)48,557
- Last updated2007-06-26 v1
- Checksum6B624B96A94E1678
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CU179680 EMBL· GenBank· DDBJ | CAL58845.1 EMBL· GenBank· DDBJ | Genomic DNA |