A5I4Z9 · ISCS_CLOBH
- ProteinCysteine desulfurase IscS
- GeneiscS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids397 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.
Catalytic activity
- [sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine
Cofactor
Pathway
Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 72-73 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 152 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 180 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 200-202 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 238 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Active site | 328 | Cysteine persulfide intermediate | |||
Binding site | 328 | [2Fe-2S] cluster (UniProtKB | ChEBI); ligand shared with IscU; via persulfide group | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | cysteine desulfurase activity | |
Molecular Function | metal ion binding | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | [2Fe-2S] cluster assembly | |
Biological Process | amino acid metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCysteine desulfurase IscS
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium
Accessions
- Primary accessionA5I4Z9
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000019405 | 1-397 | Cysteine desulfurase IscS | ||
Modified residue | 203 | N6-(pyridoxal phosphate)lysine | |||
Interaction
Subunit
Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.
Structure
Sequence
- Sequence statusComplete
- Length397
- Mass (Da)44,107
- Last updated2007-06-26 v1
- Checksum30E852D6A7DE596A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000727 EMBL· GenBank· DDBJ | ABS37989.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AM412317 EMBL· GenBank· DDBJ | CAL84126.1 EMBL· GenBank· DDBJ | Genomic DNA |