A5I4Z9 · ISCS_CLOBH

Function

function

Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.

Catalytic activity

Cofactor

pyridoxal 5'-phosphate (UniProtKB | Rhea| CHEBI:597326 )

Pathway

Cofactor biosynthesis; iron-sulfur cluster biosynthesis.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site72-73pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Binding site152pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Binding site180pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Binding site200-202pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Binding site238pyridoxal 5'-phosphate (UniProtKB | ChEBI)
Active site328Cysteine persulfide intermediate
Binding site328[2Fe-2S] cluster (UniProtKB | ChEBI); ligand shared with IscU; via persulfide group

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Functioncysteine desulfurase activity
Molecular Functionmetal ion binding
Molecular Functionpyridoxal phosphate binding
Biological Process[2Fe-2S] cluster assembly
Biological Processamino acid metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cysteine desulfurase IscS
  • EC number

Gene names

    • Name
      iscS
    • Ordered locus names
      CBO2568, CLC_2440

Organism names

Accessions

  • Primary accession
    A5I4Z9
  • Secondary accessions
    • A7G666

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_10000194051-397Cysteine desulfurase IscS
Modified residue203N6-(pyridoxal phosphate)lysine

Interaction

Subunit

Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    397
  • Mass (Da)
    44,107
  • Last updated
    2007-06-26 v1
  • Checksum
    30E852D6A7DE596A
MNKQVYMDYSATTYTKPEVLEEMLPFFTENFGNPSSLYSFSDKTKKAVNLARERVSKALNAEKNEIFFTSGGSEADNWAIKGIAYANKKKGNHIITTKIEHHAILHTAQFLEKEGFKVTYLPVDEEGFVSVEDIKNAITDETILVSVMFANNEIGTIEPIKEIGELCKEKNIYFHTDAVQAIGHVDIDVKDMNIDLLSMSAHKFYGPKGVGALYIKNGVKIQNLIHGGGQERGKRASTENTAGIVGLGKAIELAMENMPEENEKLSNLRGRLIRGIEARIPEVKLNGPKDMSRRLPNNVNFSFIGIEGETLLLDLDMNGIFGSTGSACASASLDPSHVLLSIGLPHETAHGSLRLSLGAKNTEEDIDYVLEVLPKIIKQRREMSPLWEDYMKNKEEK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000727
EMBL· GenBank· DDBJ
ABS37989.1
EMBL· GenBank· DDBJ
Genomic DNA
AM412317
EMBL· GenBank· DDBJ
CAL84126.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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