A5I365 · MOAA_CLOBH
- ProteinGTP 3',8-cyclase
- GenemoaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids319 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.
Catalytic activity
- GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H+
Cofactor
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.
Pathway
Cofactor biosynthesis; molybdopterin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 13 | GTP (UniProtKB | ChEBI) | |||
Binding site | 20 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 24 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 26 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 27 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 63 | GTP (UniProtKB | ChEBI) | |||
Binding site | 67 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 94 | GTP (UniProtKB | ChEBI) | |||
Binding site | 118 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 155 | GTP (UniProtKB | ChEBI) | |||
Binding site | 189 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 249 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | |||
Binding site | 252 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | |||
Binding site | 254-256 | GTP (UniProtKB | ChEBI) | |||
Binding site | 266 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cyclic pyranopterin monophosphate synthase activity | |
Molecular Function | GTP 3',8'-cyclase activity | |
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | Mo-molybdopterin cofactor biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGTP 3',8-cyclase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium
Accessions
- Primary accessionA5I365
- Secondary accessions
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000054186 | 1-319 | GTP 3',8-cyclase | ||
Interaction
Subunit
Monomer and homodimer.
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length319
- Mass (Da)36,402
- Last updated2007-06-26 v1
- Checksum1475CF50B29D90DF
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000727 EMBL· GenBank· DDBJ | ABS38496.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AM412317 EMBL· GenBank· DDBJ | CAL83482.1 EMBL· GenBank· DDBJ | Genomic DNA |