A5I0T6 · SYE_CLOBH
- ProteinGlutamate--tRNA ligase
- GenegltX
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids485 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).
Catalytic activity
- tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 108 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 110 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 135 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 137 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 255 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | glutamate-tRNA ligase activity | |
Molecular Function | glutamine-tRNA ligase activity | |
Molecular Function | tRNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | glutaminyl-tRNA aminoacylation | |
Biological Process | glutamyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamate--tRNA ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium
Accessions
- Primary accessionA5I0T6
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000330962 | 1-485 | Glutamate--tRNA ligase | ||
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 11-21 | 'HIGH' region | |||
Motif | 252-256 | 'KMSKS' region | |||
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length485
- Mass (Da)55,743
- Last updated2007-06-26 v1
- Checksum4D4264727F64BBB2
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000727 EMBL· GenBank· DDBJ | ABS38569.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AM412317 EMBL· GenBank· DDBJ | CAL82647.1 EMBL· GenBank· DDBJ | Genomic DNA |