A5EY43 · MNME_DICNV
- ProteintRNA modification GTPase MnmE
- GenemnmE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids450 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
Cofactor
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 23 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 81 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 120 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 226-231 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NAGKSS | ||||||
Binding site | 230 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 245-251 | GTP (UniProtKB | ChEBI) | ||||
Sequence: TPQAGTT | ||||||
Binding site | 251 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 270-273 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTAG | ||||||
Binding site | 337-340 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NKAD | ||||||
Binding site | 450 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA modification GTPase MnmE
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Cardiobacteriales > Cardiobacteriaceae > Dichelobacter
Accessions
- Primary accessionA5EY43
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000060036 | 1-450 | tRNA modification GTPase MnmE | |||
Sequence: MIANETIAAIATPPGIGGVCIIRISGAQAHAIAVNITHKKNLVPRQAILSHFYDLSGEQIDQGIVLYFPAPHSFTGEDVVELQGHGGIAVAHALLSATLDFGARLAHAGEFTQRAFLNDKLDLAQAEAVADLIHARSQDALRAANRSLQGVFSQKIDALADELLRLRVYVEASLDFSEDEIDFLGEGKIREKLVDSLQKTQQLLAQSQQGQLLNDGIHLVLAGKPNAGKSSLLNALLGEERAIVTPQAGTTRDIVREDWIIDGIPVHLSDTAGLRESQDLVEQEGIRRSFDAVKRADIVLLLADGSARDNDARAEFVSLQEELHQLAPHAQFLVVYNKADLVDEQTAGDGLWISAKTGAGIEILLKKIATLAGKNQHEETVFIARKRHIHALESVEAHLQRALQQLEQFFVAELVAEELRLAHLALGTITGTVSSDDLLDEIFSGFCIGK |
Interaction
Subunit
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 216-373 | TrmE-type G | ||||
Sequence: GIHLVLAGKPNAGKSSLLNALLGEERAIVTPQAGTTRDIVREDWIIDGIPVHLSDTAGLRESQDLVEQEGIRRSFDAVKRADIVLLLADGSARDNDARAEFVSLQEELHQLAPHAQFLVVYNKADLVDEQTAGDGLWISAKTGAGIEILLKKIATLAG |
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length450
- Mass (Da)48,801
- Last updated2007-06-12 v1
- Checksum36FF79724AB144B6
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000513 EMBL· GenBank· DDBJ | ABQ13445.1 EMBL· GenBank· DDBJ | Genomic DNA |