A4XJ89 · DDL_CALS8
- ProteinD-alanine--D-alanine ligase
- Geneddl
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids364 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Cell wall formation.
Catalytic activity
- ATP + 2 D-alanine = ADP + D-alanyl-D-alanine + H+ + phosphate
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 2 magnesium or manganese ions per subunit.
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 173-228 | ATP (UniProtKB | ChEBI) | ||||
Sequence: ESEFSYPVFVKPANSGSSVGISKAKDKDELILAIHEAFLYDTKILIEQAINAREIE | ||||||
Binding site | 299 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 313 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 313 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 315 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | D-alanine-D-alanine ligase activity | |
Molecular Function | metal ion binding | |
Biological Process | cell wall organization | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameD-alanine--D-alanine ligase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacillota incertae sedis > Caldicellulosiruptorales > Caldicellulosiruptoraceae > Caldicellulosiruptor
Accessions
- Primary accessionA4XJ89
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000030434 | 1-364 | D-alanine--D-alanine ligase | |||
Sequence: MTKLKVAVLFGGVSTEHEVSIVSAKSVMQNLDKEKYEIIPIGITKEGKWLLYTGKIEDLDSKWTMYSIECFISPDRTKKALVKIKDSEATFIDIDVVFPVLHGLNGEDGTVQGLLELSGIPYVGCGVLSSALCMDKAFAKKLALLEGIPQGHFLVIYKDEYLSKKEYFIRRIESEFSYPVFVKPANSGSSVGISKAKDKDELILAIHEAFLYDTKILIEQAINAREIECAVLGNSEILVSALGEIVPSREFYSYEAKYIDGSSELIIPAKLEKQVEDEIKELAVKIYKLFECSGMARIDFFVDKETNKVYFNEVNTIPGFTSISMYPKLMEYSGIPYPQLLDKLIQLAIEKNQQKQSIKYSKEG |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 140-346 | ATP-grasp | ||||
Sequence: KKLALLEGIPQGHFLVIYKDEYLSKKEYFIRRIESEFSYPVFVKPANSGSSVGISKAKDKDELILAIHEAFLYDTKILIEQAINAREIECAVLGNSEILVSALGEIVPSREFYSYEAKYIDGSSELIIPAKLEKQVEDEIKELAVKIYKLFECSGMARIDFFVDKETNKVYFNEVNTIPGFTSISMYPKLMEYSGIPYPQLLDKLIQ |
Sequence similarities
Belongs to the D-alanine--D-alanine ligase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length364
- Mass (Da)40,890
- Last updated2007-05-29 v1
- Checksum7AEAA0179641995C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000679 EMBL· GenBank· DDBJ | ABP66974.1 EMBL· GenBank· DDBJ | Genomic DNA |