A4VUF4 · THII_STRSY
- ProteinProbable tRNA sulfurtransferase
- GenethiI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids405 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.
Catalytic activity
- [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]
- [sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H+ + L-cysteinyl-[cysteine desulfurase]
Pathway
Cofactor biosynthesis; thiamine diphosphate biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | CCA tRNA nucleotidyltransferase activity | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uracil-4 sulfurtransferase activity | |
Biological Process | thiamine biosynthetic process | |
Biological Process | thiamine diphosphate biosynthetic process | |
Biological Process | tRNA thio-modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable tRNA sulfurtransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus
Accessions
- Primary accessionA4VUF4
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000074303 | 1-405 | Probable tRNA sulfurtransferase | |||
Sequence: MNYSEIMIRYGELSTKGKNKMRFVNKLRNNIKHVLSVYPEVTVYFDRDRGHVYLNGADYQEVSASLKKIFGIQNFAPSYKIEKSVPALKEAVVEIMQTIYKEGMTFKIAARRSDHSFELDSRDLNQVLGDAVFTAIPNVQVQMKSPDITLRVEIRPDAAYISHEEIKGAGGLPVGTSGKGTLMLSGGIDSPVAGYLALKRGVEIEALHFASPPYTSPGALKKAHDLTRKLTAFGGNITFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRVREERGAMVIINGESLGQVASQTLESMQAINAVTNTPVIRPVVTMDKLEIIDIAQEIDTFDISIQPFEDCCTIFAPDRPKTNPKIKNVEQYEARMDVEGLVERAVAGIIVTEITPKEEVKDEVDSLIEDLL |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 60-165 | THUMP | ||||
Sequence: QEVSASLKKIFGIQNFAPSYKIEKSVPALKEAVVEIMQTIYKEGMTFKIAARRSDHSFELDSRDLNQVLGDAVFTAIPNVQVQMKSPDITLRVEIRPDAAYISHEE |
Sequence similarities
Belongs to the ThiI family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length405
- Mass (Da)45,238
- Last updated2007-05-29 v1
- Checksum0B1167639A6971FA
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000407 EMBL· GenBank· DDBJ | ABP89743.1 EMBL· GenBank· DDBJ | Genomic DNA |