A4SYV5 · DNLJ_POLAQ

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

167150100150200250300350400450500550600650
TypeIDPosition(s)Description
Binding site37-41NAD+ (UniProtKB | ChEBI)
Binding site86-87NAD+ (UniProtKB | ChEBI)
Binding site117NAD+ (UniProtKB | ChEBI)
Active site119N6-AMP-lysine intermediate
Binding site140NAD+ (UniProtKB | ChEBI)
Binding site177NAD+ (UniProtKB | ChEBI)
Binding site295NAD+ (UniProtKB | ChEBI)
Binding site319NAD+ (UniProtKB | ChEBI)
Binding site413Zn2+ (UniProtKB | ChEBI)
Binding site416Zn2+ (UniProtKB | ChEBI)
Binding site431Zn2+ (UniProtKB | ChEBI)
Binding site437Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • Ordered locus names
      Pnuc_1455

Organism names

Accessions

  • Primary accession
    A4SYV5

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003403651-671DNA ligase

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain594-671BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    671
  • Mass (Da)
    73,743
  • Last updated
    2007-05-15 v1
  • Checksum
    27C6F953067C0E10
MSSNSPTNLADRYAFLQAELARLEHAYYVLDNPLLPDIEYDRLYRELLDIEAAHPQWVTSESLSQRVGGTALKEFDSVTHAVPMLSLNNAFEDSELIAFDRRCREALHVEHVAYAGELKFDGLAISLRYENGTLVTAATRGDGASGEDVTANIKTIRAIPLKLTGKNIPEILEVRGEVFMYLKDFEKMNRQAAELGEKEFANPRNAAAGSLRQLDSKITAKRPLSFFAYGLGALEPQSWLPKTHEELLNAYVELGLPVCSERRVLHSVEEILAFYNEIGAKRDSLPYDIDGVVYKVNSFAEQAKLGFVSRAPRFALAHKYPAQEALTTVLGIDVQVGRTGAITPVARLAPVEVGGVTVTNATLHNEDEVKRKDVRIGDTVSVRRAGDVIPEVVSVIKERRPSDAKEFVMPSRCPVCDSHIERLADEAVARCSGGLFCGAQRKQALIHFAHRRALDIEGLGEKIVDQLVDQNLVRTPADLYRLGFTALANLERMGEKSADNLIQAINQSRNTTLARFIFALGIRHVGETTAKDLANHYQSMHALMDASVEELLTVKDVGPVVADSITSFMQEAHNREVIEQLLASGMQLSVEEKIISAAVFGKTFVLTGTFPTMTRDEAKDLLEKAGAKVAGSVSKKTDYVVAGTDAGSKLAKAEELGVPVIDEEEMLNLLK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000655
EMBL· GenBank· DDBJ
ABP34669.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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