A4IX14 · MNME_FRATW
- ProteintRNA modification GTPase MnmE
- GenemnmE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids450 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
Cofactor
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 23 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 79 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 118 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 224 | K+ (UniProtKB | ChEBI) | |||
Binding site | 224-229 | GTP (UniProtKB | ChEBI) | |||
Binding site | 228 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 243 | K+ (UniProtKB | ChEBI) | |||
Binding site | 243-249 | GTP (UniProtKB | ChEBI) | |||
Binding site | 245 | K+ (UniProtKB | ChEBI) | |||
Binding site | 248 | K+ (UniProtKB | ChEBI) | |||
Binding site | 249 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 268-271 | GTP (UniProtKB | ChEBI) | |||
Binding site | 450 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA modification GTPase MnmE
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Thiotrichales > Francisellaceae > Francisella
Accessions
- Primary accessionA4IX14
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000060046 | 1-450 | tRNA modification GTPase MnmE | ||
Interaction
Subunit
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 214-374 | TrmE-type G | |||
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length450
- Mass (Da)49,796
- Last updated2007-05-01 v1
- Checksum095E6F17E66AE4F9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000608 EMBL· GenBank· DDBJ | ABO46466.1 EMBL· GenBank· DDBJ | Genomic DNA |