A4IX14 · MNME_FRATW

Function

function

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.

Cofactor

K+ (UniProtKB | Rhea| CHEBI:29103 )

Note: Binds 1 potassium ion per subunit.

Features

Showing features for binding site.

145050100150200250300350400450
TypeIDPosition(s)Description
Binding site23(6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site79(6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site118(6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site224K+ (UniProtKB | ChEBI)
Binding site224-229GTP (UniProtKB | ChEBI)
Binding site228Mg2+ (UniProtKB | ChEBI)
Binding site243K+ (UniProtKB | ChEBI)
Binding site243-249GTP (UniProtKB | ChEBI)
Binding site245K+ (UniProtKB | ChEBI)
Binding site248K+ (UniProtKB | ChEBI)
Binding site249Mg2+ (UniProtKB | ChEBI)
Binding site268-271GTP (UniProtKB | ChEBI)
Binding site450(6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionmetal ion binding
Biological ProcesstRNA methylation
Biological ProcesstRNA wobble uridine modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA modification GTPase MnmE
  • EC number

Gene names

    • Name
      mnmE
    • Synonyms
      trmE
    • Ordered locus names
      FTW_0557

Organism names

Accessions

  • Primary accession
    A4IX14

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000600461-450tRNA modification GTPase MnmE

Interaction

Subunit

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain214-374TrmE-type G

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    450
  • Mass (Da)
    49,796
  • Last updated
    2007-05-01 v1
  • Checksum
    095E6F17E66AE4F9
MYTKDTIVAIATPQGNGGIGIIRISGIDALAIAEKLTKKQLKPRYATFCNVYNDNEIIDHGIVIFFKAPLSYTGEDVVEIQAHGNPFILNLIIKAALNCGARMAKAGEFTERAFLNNKLDLAQAEAVADIINASSEIAAKSAAKSLQGDFSKEINNLLEKLIYLRMYVEASIDFPEEEINFLEDQKIHSSLEEIYKVILAVKNSCKQGVILAEGITLILVGKPNAGKSSLLNALAGKESAIVTSIAGTTRDIVKEHIQINGVPMHIIDTAGLRNSDDIIESEGIKRAIKKIQEADQVLFVTDDYTNSQVKFSDIKEIIPEFYDQIPKDIDITYVHNKIDLLKEVPHNHANHIYISAENNIGIDKLKEHILNKVGYTNQNESIYTARERHVTAINNAFEHIKLAREQLELGNGELLAEELLIVQEYLNSITGEFSSDDLLGEIFSSFCIGK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000608
EMBL· GenBank· DDBJ
ABO46466.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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