A4FV12 · A4FV12_BOVIN
- ProteinSUMO-activating enzyme subunit 2
- GeneUBA2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids640 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Pathway
Protein modification; protein sumoylation.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 24-29 | ATP (UniProtKB | ChEBI) | |||
Binding site | 48 | ATP (UniProtKB | ChEBI) | |||
Binding site | 56-59 | ATP (UniProtKB | ChEBI) | |||
Binding site | 72 | ATP (UniProtKB | ChEBI) | |||
Binding site | 95-96 | ATP (UniProtKB | ChEBI) | |||
Binding site | 117-122 | ATP (UniProtKB | ChEBI) | |||
Binding site | 158 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 161 | Zn2+ (UniProtKB | ChEBI) | |||
Active site | 173 | Glycyl thioester intermediate | |||
Binding site | 441 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 444 | Zn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleoplasm | |
Cellular Component | SUMO activating enzyme complex | |
Molecular Function | ATP binding | |
Molecular Function | magnesium ion binding | |
Molecular Function | protein heterodimerization activity | |
Molecular Function | small protein activating enzyme binding | |
Molecular Function | SUMO activating enzyme activity | |
Molecular Function | SUMO binding | |
Molecular Function | ubiquitin-like protein conjugating enzyme binding | |
Biological Process | positive regulation of protein sumoylation | |
Biological Process | protein sumoylation |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSUMO-activating enzyme subunit 2
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionA4FV12
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for cross-link.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Cross-link | 164 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) | |||
Cross-link | 190 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | |||
Cross-link | 236 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate | |||
Cross-link | 257 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate | |||
Cross-link | 420 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternate | |||
Cross-link | 623 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) | |||
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Heterodimer of SAE1 and UBA2/SAE2. The heterodimer corresponds to the two domains that are encoded on a single polypeptide chain in ubiquitin-activating enzyme E1. Interacts with UBE2I.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 13-441 | THIF-type NAD/FAD binding fold | |||
Region | 202-233 | Disordered | |||
Compositional bias | 208-233 | Basic and acidic residues | |||
Region | 287-306 | Disordered | |||
Compositional bias | 288-306 | Polar residues | |||
Domain | 452-538 | Ubiquitin/SUMO-activating enzyme ubiquitin-like | |||
Domain | 549-630 | SUMO-activating enzyme subunit 2 C-terminal | |||
Region | 551-628 | Disordered | |||
Compositional bias | 563-586 | Polar residues | |||
Compositional bias | 601-628 | Basic and acidic residues | |||
Sequence similarities
Belongs to the ubiquitin-activating E1 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length640
- Mass (Da)71,031
- Last updated2007-04-17 v1
- Checksum4B1946642E4AE5DA
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0AAA9SHV8 | A0AAA9SHV8_BOVIN | UBA2 | 636 | ||
A0AAA9SUH8 | A0AAA9SUH8_BOVIN | UBA2 | 607 | ||
A0A3Q1MG31 | A0A3Q1MG31_BOVIN | UBA2 | 583 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 208-233 | Basic and acidic residues | |||
Compositional bias | 288-306 | Polar residues | |||
Compositional bias | 563-586 | Polar residues | |||
Compositional bias | 601-628 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC123590 EMBL· GenBank· DDBJ | AAI23591.1 EMBL· GenBank· DDBJ | mRNA |