A4FBV6 · MSHC_SACEN
- ProteinL-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
- GenemshC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids412 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins.
Catalytic activity
- 1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside + L-cysteine + ATP = 1D-myo-inositol 2-(L-cysteinylamino)-2-deoxy-alpha-D-glucopyranoside + AMP + diphosphate + H+
Cofactor
Note: Binds 1 zinc ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 43 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 43-46 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | |||
Binding site | 58 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | |||
Binding site | 81-83 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | |||
Binding site | 227 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | |||
Binding site | 231 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 249-251 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | |||
Binding site | 256 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 283 | L-cysteinyl-5'-AMP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | cysteine-glucosaminylinositol ligase activity | |
Molecular Function | cysteine-tRNA ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | cysteinyl-tRNA aminoacylation | |
Biological Process | mycothiol biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameL-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
- EC number
- Short namesL-Cys:GlcN-Ins ligase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Saccharopolyspora
Accessions
- Primary accessionA4FBV6
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000400480 | 1-412 | L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase | ||
Interaction
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 45-55 | 'HIGH' region | |||
Motif | 187-192 | 'ERGGDP' region | |||
Motif | 289-293 | 'KMSKS' region | |||
Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length412
- Mass (Da)45,382
- Last updated2007-04-17 v1
- MD5 Checksum640B1A4A5291E1924EE555FE22A78F69
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AM420293 EMBL· GenBank· DDBJ | CAM01531.1 EMBL· GenBank· DDBJ | Genomic DNA |