A3SLS0 · HPAAL_ROSNI
- ProteinHydroxypyruvate/pyruvate aldolase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids257 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Aldolase which can catalyze in vitro the aldolisation reaction between hydroxypyruvate (HPA) or pyruvate (PA) and D-glyceraldehyde (D-GA) (Ref.2). The condensation of hydroxypyruvate and D-glyceraldehyde produces 2-dehydro-D-gluconate as the major product (Ref.2). The condensation of pyruvate and D-glyceraldehyde produces 2-dehydro-3-deoxy-L-galactonate as the major product and 2-dehydro-3-deoxy-D-gluconate (Ref.2).
Catalytic activity
- 3-hydroxypyruvate + D-glyceraldehyde = 2-dehydro-D-gluconate
- D-glyceraldehyde + pyruvate = 2-dehydro-3-deoxy-L-galactonate
- 2-dehydro-3-deoxy-D-gluconate = D-glyceraldehyde + pyruvate
Cofactor
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 48 | Proton acceptor | ||||
Sequence: H | ||||||
Site | 73 | Transition state stabilizer | ||||
Sequence: R | ||||||
Site | 87 | Increases basicity of active site His | ||||
Sequence: D | ||||||
Binding site | 152 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 178 | a divalent metal cation (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | aldehyde-lyase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHydroxypyruvate/pyruvate aldolase
- EC number
- Short namesHPA/PA aldolase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodobacterales > Roseobacteraceae > Roseovarius
Accessions
- Primary accessionA3SLS0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000460958 | 1-257 | Hydroxypyruvate/pyruvate aldolase | |||
Sequence: MPAPTNTFKSALASGDLLLGCWAGFADPYATEVLATAGYDWLVIDGEHAPNDLRSISAQLAVLRGQHSHPVVRLPMGEPWLIKQVLDAGAQTLLIPMVESAEEARDLVRAMRYPPDGIRGSGAALARASHFADIPDYIATANDQMCLLVQVETRAGIDALDEILAVDGVDGVFIGPSDLAADMGHMGDASQADVAETIEDALARIRTAGRAAGILALDDATIRRYRDWGANFLAVGIDVVMLAQTARQTLAKWRTDD |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length257
- Mass (Da)27,418
- Last updated2007-04-03 v1
- Checksum270D89A0E50E5940
Keywords
- Technical term