A3SHP3 · A3SHP3_ROSNI

Function

function

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site191-194CoA (UniProtKB | ChEBI)
Binding site309CoA (UniProtKB | ChEBI)
Binding site385-387ATP (UniProtKB | ChEBI)
Binding site409-414ATP (UniProtKB | ChEBI)
Binding site500ATP (UniProtKB | ChEBI)
Binding site515ATP (UniProtKB | ChEBI)
Binding site523CoA (UniProtKB | ChEBI)
Binding site526ATP (UniProtKB | ChEBI)
Binding site537Mg2+ (UniProtKB | ChEBI)
Binding site539Mg2+ (UniProtKB | ChEBI)
Binding site542Mg2+ (UniProtKB | ChEBI)
Binding site584CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionacetate-CoA ligase activity
Molecular FunctionAMP binding
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Biological Processacetyl-CoA biosynthetic process from acetate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acetyl-coenzyme A synthetase
  • EC number
  • Short names
    AcCoA synthetase
    ; Acs
  • Alternative names
    • Acetate--CoA ligase
    • Acyl-activating enzyme

Gene names

    • Name
      acsA
    • ORF names
      ISM_01255

Organism names

Accessions

  • Primary accession
    A3SHP3

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue609N6-acetyllysine

Post-translational modification

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.

Keywords

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain23-80Acetyl-coenzyme A synthetase N-terminal
Domain82-469AMP-dependent synthetase/ligase
Domain531-609AMP-binding enzyme C-terminal

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    649
  • Mass (Da)
    71,845
  • Last updated
    2007-04-03 v1
  • Checksum
    D02E44055AB61559
MSDTIHPPSAEFAENAHIDAARYRALYDASLRDPEAFWQEHGQRIDWIKPFTKVKDVNFDLGNVSIKWFEDGTLNVAANCIDRHLASRGDQTAIIWEPDSPDEEALHISYRELHARTCKMANALKELGVTRGDRVVLYMPMIPEAAYAMLACARIGAIHSVVFGGFSSDALAARVNGSEAKVVVTADEAPRGGRNTPLKTNADKALDGVSHDCKLLVVQRTGGDIPMQEGRDHWLHDLEAKVADDCPPEEMNAEDPLFILYTSGSTGAPKGVVHTSGGYLTWASMTHELVFDYHDGDIYWCTADVGWVTGHSYIVYGPLANGATTLMFEGVPTYPDASRFWQVCEKHKVNQFYTAPTAIRALMGKGREFVDRCDLSSLKLLGTVGEPINPEAWNWYNDVVGQGRCPIVDTWWQTETGGHLMTPLPGATATKPGSCTTPFFGVEPVVLDAQSGEEITSTEAEGVLCIKSSWPGQMRSVWGDHERFEKTYFSDYKGYYFTGDGCRRDKDGYYWITGRVDDVINVSGHRMGTAEVESALVAHPKVSEAAVVGYPHDVKGQGIYCYVTLMAGEEYTDDLRKELRDWVRKEIGPIASPDLIQWAPGLPKTRSGKIMRRILRKIAEDDFGSLGDTSTLAEPAVVDDLIENRMNRS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AALY01000001
EMBL· GenBank· DDBJ
EAP76874.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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