A3MWK6 · DHE4_PYRCJ

Function

function

Catalyzes the reversible oxidative deamination of L-glutamate to 2-oxoglutarate and ammonia, thereby playing a key role at the intersection of the carbon and nitrogen metabolic pathways. Shows a high preference for NADP+/NADPH as the acceptor/donor over NAD+/NADH. May function in vivo in the synthetic direction. Also catalyzes at very low rates the oxidative deamination of L-2-aminobutyrate, and the reductive amination of 2-oxovalerate and 2-oxobutyrate.

Catalytic activity

Activity regulation

Is not regulated allosterically. Activity is inhibited in the presence of high ionic strength; the inhibitory effect of KCl is slightly higher than that of NaCl.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.035 mMNADP+50
3.4 mML-glutamate50
0.017 mMNADPH50
1.7 mM2-oxoglutarate50
2.2 mMammonia50
kcat is 13 sec-1 for oxidative deamination of L-glutamate, and 95 sec-1 for reductive amination of 2-oxoglutarate (at 50 degrees Celsius).

pH Dependence

Optimum pH is 9.5 for oxidative deamination, and 9.0 for reductive amination. Retains more than 80% of its activity after incubation for 30 minutes at pH 4.5-9.5.

Temperature Dependence

Optimum temperature is 90 degrees Celsius for oxidative deamination. Retains full activity after incubation for 10 minutes at temperatures up to 90 degrees Celsius.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site70substrate
Binding site94substrate
Active site106Proton donor
Site146Important for catalysis
Binding site190NADP+ (UniProtKB | ChEBI)
Binding site221NADP+ (UniProtKB | ChEBI)
Binding site354substrate

GO annotations

AspectTerm
Molecular Functionglutamate dehydrogenase (NAD+) activity
Molecular Functionglutamate dehydrogenase (NADP+) activity
Molecular FunctionNADP+ binding
Molecular FunctionNADPH binding
Biological Process2-oxoglutarate metabolic process
Biological Processglutamate catabolic process
Biological Processglutamate metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADP(+)-dependent glutamate dehydrogenase
  • EC number
  • Short names
    NADP-GDH

Gene names

    • Ordered locus names
      Pcal_1606

Organism names

Accessions

  • Primary accession
    A3MWK6

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004322281-421NADP+-dependent glutamate dehydrogenase

Expression

Induction

Is constitutively expressed.

Interaction

Subunit

Homohexamer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    421
  • Mass (Da)
    46,610
  • Last updated
    2007-04-03 v1
  • Checksum
    DECEAEB7942AE122
MSQNGQFLEYTLQVIRRGVEMGGFPEDFYKLLSRPKRIIQVSIPVKMDNGSYEVFEGYRVQHNDALGPFKGGIRFHPEVTLADDIALAMLMTLKNSLAGLPYGGAKGAVRVDPRRLSRRELEELARGYARAVAPLIGEQLDIPAPDVGTDSQVMAWMVDEYSKLAGRNAPAVFTSKPPELWGNPVREYSTGFGVAVAAREVAKRLWGGIEGKTVAVHGAGNTGAWAAYWLEKMGAKVVAISDTRGTVVNKAGIPGEQILKVYMEKKRDKSATVLALEGEKIADSNASLYQDVDILVPAAIENVVREDNVGLVRARLVVEGANGPTTPGAERRLYERGVVVVPDILANAGGVIMSYLEWVENLQWLFWDEEETRRRLEAIMSNNVARVYARWEKEKSWTMRDAAVVTALERIYNAMKTRGWI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000561
EMBL· GenBank· DDBJ
ABO09023.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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