A3LX75 · VPS27_PICST
- ProteinVacuolar protein sorting-associated protein 27
- GeneVPS27
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids732 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 193 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 196 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 209 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 212 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 217 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 220 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 239 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 242 | Zn2+ 2 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endosome membrane | |
Cellular Component | ESCRT-0 complex | |
Molecular Function | metal ion binding | |
Molecular Function | phosphatidylinositol-3-phosphate binding | |
Molecular Function | ubiquitin binding | |
Biological Process | protein targeting to vacuole | |
Biological Process | protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway |
Keywords
- Ligand
Names & Taxonomy
Protein names
- Recommended nameVacuolar protein sorting-associated protein 27
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Debaryomycetaceae > Scheffersomyces
Accessions
- Primary accessionA3LX75
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Endosome membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000292522 | 1-732 | Vacuolar protein sorting-associated protein 27 | ||
Interaction
Subunit
Component of the ESCRT-0 complex composed of HSE1 and VPS27.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, zinc finger, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 23-166 | VHS | |||
Zinc finger | 187-247 | FYVE-type; atypical | |||
Region | 245-272 | Disordered | |||
Compositional bias | 246-263 | Polar residues | |||
Domain | 271-290 | UIM 1 | |||
Region | 287-316 | Disordered | |||
Domain | 313-332 | UIM 2 | |||
Region | 329-357 | Disordered | |||
Region | 483-732 | Disordered | |||
Compositional bias | 491-570 | Polar residues | |||
Compositional bias | 590-633 | Polar residues | |||
Compositional bias | 686-717 | Polar residues | |||
Domain
The FYVE domain is involved in the binding to phosphatidylinositol 3-phosphate (PtdIns3P) which is required for the association to endosomal membranes.
Both IUM domains are necessary for efficient binding to ubiquitin.
Sequence similarities
Belongs to the VPS27 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length732
- Mass (Da)83,145
- Last updated2007-07-24 v2
- MD5 Checksum8939CFF5F72216F7539E2DA57B47C4C1
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 246-263 | Polar residues | |||
Compositional bias | 491-570 | Polar residues | |||
Compositional bias | 590-633 | Polar residues | |||
Compositional bias | 686-717 | Polar residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000500 EMBL· GenBank· DDBJ | ABN67754.2 EMBL· GenBank· DDBJ | Genomic DNA |