A3LUK5 · DUS3_PICST
- ProteintRNA-dihydrouridine(47) synthase [NAD(P)(+)]
- GeneDUS3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids613 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs (By similarity).
Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation (By similarity).
Catalyzes the synthesis of dihydrouridine in some mRNAs, thereby affecting their translation (By similarity).
Catalytic activity
- 5,6-dihydrouridine47 in tRNA + NAD+ = H+ + NADH + uridine47 in tRNAThis reaction proceeds in the backward direction.
- a 5,6-dihydrouridine in mRNA + NAD+ = a uridine in mRNA + H+ + NADHThis reaction proceeds in the backward direction.
- a 5,6-dihydrouridine in mRNA + NADP+ = a uridine in mRNA + H+ + NADPHThis reaction proceeds in the backward direction.
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 250-252 | FMN (UniProtKB | ChEBI) | ||||
Sequence: PLT | ||||||
Binding site | 305 | FMN (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Active site | 337 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 377 | FMN (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 408 | FMN (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 456-458 | FMN (UniProtKB | ChEBI) | ||||
Sequence: NGD | ||||||
Binding site | 480-481 | FMN (UniProtKB | ChEBI) | ||||
Sequence: AR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | metal ion binding | |
Molecular Function | mRNA dihydrouridine synthase activity | |
Molecular Function | tRNA-dihydrouridine47 synthase activity | |
Biological Process | mRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine(47) synthase [NAD(P)(+)]
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Debaryomycetaceae > Scheffersomyces
Accessions
- Primary accessionA3LUK5
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000330247 | 1-613 | tRNA-dihydrouridine47 synthase [NAD(P)+] | |||
Sequence: MTAGEKRTSEALDQPESKKVHVERGMAAIKPEFIVQKDRIVDSFDDDEAEGGERGVEEGKSGKGGKKKRRGQNKNRDLKQKREEIRLCTSLLDPDNIKTCAYGPEQCRSTHNVEEYLASKPVDIEGICPVFRAIGYCPAGLKCRWLQSHYDKETRKLIKDLGRTEASKIELNYEVNNVSHEARGKLRKKQYDFAIAGKVIEYIDSTVQNDENIANAKEQRKNNEATYVDAPYKIAEKKRLDFRNAKIVSPLTTVGNLPYRRLMKKLGADITYSEMALSVPLLQATNAEWALPKAHRTEYPGYGVQIATSKHWAAAKVAEIISRETEHVSELNLNCGCPIDLLYRQGQGSALLEQPARLVRILKAMNASSGDIPVTVKIRTGSKENKNTAKTLVERLLAENDVAAITLHGRSRQQRYTKEADWNYIAEVGQVVQQWNDKKEENKDSRDTQRTCFVGNGDVFSHVDWYNAVNTDGIDSVMVARGALIKPWIFEEVEAQQYLDKTATERLDILKTFSDYALEHWGTDEYGVGLARRFMCEFLSFTHRYIPLGILERLPPKINERPPQWKGRNEMETLLGSTDYKDWIKITEMFLGKSGDDFVFTPKHKSNSYEKSN |
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, zinc finger.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-24 | Disordered | ||||
Sequence: MTAGEKRTSEALDQPESKKVHVER | ||||||
Region | 40-78 | Disordered | ||||
Sequence: IVDSFDDDEAEGGERGVEEGKSGKGGKKKRRGQNKNRDL | ||||||
Zinc finger | 88-111 | C3H1-type 1 | ||||
Sequence: CTSLLDPDNIKTCAYGPEQCRSTH | ||||||
Zinc finger | 128-149 | C3H1-type 2 | ||||
Sequence: CPVFRAIGYCPAGLKCRWLQSH |
Sequence similarities
Belongs to the Dus family. Dus3 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length613
- Mass (Da)69,682
- Last updated2007-04-03 v1
- Checksum2F1C257CCA755FB8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000498 EMBL· GenBank· DDBJ | ABN66252.1 EMBL· GenBank· DDBJ | Genomic DNA |