A3GGE9 · DBP4_PICST
- ProteinATP-dependent RNA helicase DBP4
- GeneDBP4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids765 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
ATP-dependent RNA helicase required for ribosome biogenesis. Involved in the release of U14 snoRNA in pre-ribosomal complexes. Required for pre-rRNA cleavage at site A2 (By similarity).
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleolus | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Biological Process | rRNA processing |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent RNA helicase DBP4
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Debaryomycetaceae > Scheffersomyces
Accessions
- Primary accessionA3GGE9
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000285141 | 1-765 | ATP-dependent RNA helicase DBP4 | |||
Sequence: MAKPRRNNKNKKGQSRSQAREKEEAELLKLQERIDQYDPAVDEKSISQFSDLPITQETLRGLNESSFMSLTDIQKKTIPIALKGEDLMGTARTGSGKTLAFLIPVVESLIRNKITEHDGLAALIVSPTRELAVQTFEVLTKIGKYNTFSAGLVTGGKDVQYEKERVSRMNILVGTPGRISQHLNEAVGMETSNLQVLVLDEADRCLDMGFKKQIDNIVGHLPPTRQTLLFSATVSDSVKDLARLSLTNPKRIGVSSDQDVSATPESLDQYYIRIPLDEKLDVLWSFIKSHLKSKILVFFSSSKQVQYAYETFRTLQPGISLLKLYGRHKQTSRLETTVKFTQAQYACLFATDIVARGLDFPAIDWVVQVDCPEDAVTYVHRVGRAARFGRQGKSLLMLLPSEEEGMLKRLENNKIEPKFMNIKQKSKKSIRPQLQSLCFKDPMIKNLGQRAFISYYKSVYIQKDKDVFKVEELPSEKYAASLGLPGAPKIKIKGGSSSKEKKNASRQLVALSKTNEDGESVENEASKVRTKYDRMFERKNQNVLSEHYLKLNGSQTKEDEDEEEEDDFMSVKRKDHELTEVELPDLTIPVSKRQAKKALSKKLSISTKGNPTKLKFDDEGVAHAIYELEDEEDFIKRGDAKEQKKAFVSRETEVMNEADEEDKIMAKEKRQEKKRKRKEVEKRMRDEELQSGDDEETVYTLGGDVDLERDMEYSTDEEEQPQNKKPKWFENDKHNRKNPVNHIVEIDEPETLEDLESLTARLLQH |
Interaction
Subunit
Interacts with the U3 and U14 snoRNAs. Associates with pre-ribosomal complexes (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, motif, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-24 | Disordered | ||||
Sequence: MAKPRRNNKNKKGQSRSQAREKEE | ||||||
Motif | 47-75 | Q motif | ||||
Sequence: SQFSDLPITQETLRGLNESSFMSLTDIQK | ||||||
Domain | 78-252 | Helicase ATP-binding | ||||
Sequence: IPIALKGEDLMGTARTGSGKTLAFLIPVVESLIRNKITEHDGLAALIVSPTRELAVQTFEVLTKIGKYNTFSAGLVTGGKDVQYEKERVSRMNILVGTPGRISQHLNEAVGMETSNLQVLVLDEADRCLDMGFKKQIDNIVGHLPPTRQTLLFSATVSDSVKDLARLSLTNPKRI | ||||||
Motif | 200-203 | DEAD box | ||||
Sequence: DEAD | ||||||
Domain | 266-438 | Helicase C-terminal | ||||
Sequence: SLDQYYIRIPLDEKLDVLWSFIKSHLKSKILVFFSSSKQVQYAYETFRTLQPGISLLKLYGRHKQTSRLETTVKFTQAQYACLFATDIVARGLDFPAIDWVVQVDCPEDAVTYVHRVGRAARFGRQGKSLLMLLPSEEEGMLKRLENNKIEPKFMNIKQKSKKSIRPQLQSLC | ||||||
Region | 493-526 | Disordered | ||||
Sequence: KGGSSSKEKKNASRQLVALSKTNEDGESVENEAS | ||||||
Compositional bias | 501-516 | Polar residues | ||||
Sequence: KKNASRQLVALSKTNE | ||||||
Region | 552-576 | Disordered | ||||
Sequence: NGSQTKEDEDEEEEDDFMSVKRKDH | ||||||
Compositional bias | 641-669 | Basic and acidic residues | ||||
Sequence: KEQKKAFVSRETEVMNEADEEDKIMAKEK | ||||||
Region | 641-735 | Disordered | ||||
Sequence: KEQKKAFVSRETEVMNEADEEDKIMAKEKRQEKKRKRKEVEKRMRDEELQSGDDEETVYTLGGDVDLERDMEYSTDEEEQPQNKKPKWFENDKHN | ||||||
Compositional bias | 716-735 | Basic and acidic residues | ||||
Sequence: DEEEQPQNKKPKWFENDKHN |
Domain
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities
Belongs to the DEAD box helicase family. DDX10/DBP4 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length765
- Mass (Da)87,257
- Last updated2010-01-19 v2
- ChecksumE581A5E1A0AF8379
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 501-516 | Polar residues | ||||
Sequence: KKNASRQLVALSKTNE | ||||||
Compositional bias | 641-669 | Basic and acidic residues | ||||
Sequence: KEQKKAFVSRETEVMNEADEEDKIMAKEK | ||||||
Compositional bias | 716-735 | Basic and acidic residues | ||||
Sequence: DEEEQPQNKKPKWFENDKHN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAVQ01000001 EMBL· GenBank· DDBJ | EAZ63509.2 EMBL· GenBank· DDBJ | Genomic DNA |