A3DM85 · PRIS_STAMF
- ProteinDNA primase small subunit PriS
- GenepriS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids377 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 99 | |||||
Sequence: D | ||||||
Active site | 101 | |||||
Sequence: D | ||||||
Active site | 274 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Cellular Component | primosome complex | |
Molecular Function | DNA primase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA primase small subunit PriS
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Desulfurococcales > Desulfurococcaceae > Staphylothermus
Accessions
- Primary accessionA3DM85
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_1000132348 | 1-377 | DNA primase small subunit PriS | |||
Sequence: MSRQESSNKFFIKKILREYYSRKPLEEPLYIHKREIAIHSLEDEAYIRHLSFPSITHLYNFILNEKTPLHLYYSSAYYESPSVNKMELKGWIGSDLMFDLDSDHYPGCDKILSICIEENTIYDGKIKTCPKTESKPVIHPLIKTECIQKAYRDALRIKYILEDELGLKNIKIYFSGNRGFHVKVIDEKIWDLESDERREIASYISLENFDINKLFPVIGKRKKYVIITRNEHGIRKRVLDYVIKNNIVNGNELFIKLPLKLLEETINDLTIPIDIVVTMDISRLSRFGNSINGKSGLITKLIEPLDNYEFDINDFCPWSGNIVVKPLIDISGLQVFDEKIDLKRGVKKILDSKIAVYLTLKGIVKIISDEKLVIKNV |
Interaction
Subunit
Heterodimer of a small subunit (PriS) and a large subunit (PriL).
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length377
- Mass (Da)43,866
- Last updated2007-03-20 v1
- Checksum219CC7886169CABD
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000575 EMBL· GenBank· DDBJ | ABN69745.1 EMBL· GenBank· DDBJ | Genomic DNA |