A3DDX7 · DAPDH_ACET2

Function

function

Catalyzes the reversible NADPH-dependent reductive amination of L-2-amino-6-oxopimelate, the acyclic form of L-tetrahydrodipicolinate, to generate the meso compound, D,L-2,6-diaminopimelate. Probably plays a role in lysine biosynthesis. Exhibits a high substrate specificity for meso-2,6-diaminopimelate (m-DAP), since the activity with L,L-2,6-diaminopimelate is less than 5% of the activity observed with m-DAP. Can use NAD+ only very poorly since the activity observed in the presence of NAD+ is about 0.3% of that with NADP+.

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.07 mMmeso-2,6-diaminoheptanedioate10.530
0.13 mMNADP+10.530
0.09 mML-2-amino-6-oxoheptanedioate10.530
0.21 mMNADPH10.530
195 mMammonia10.530
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
15.45 μmol/min/mg10.530for the reductive amination of L-2-amino-6-oxopimelate
0.24 μmol/min/mg10.530for the oxidative deamination of meso-2,6-diaminopimelate

Temperature Dependence

Optimum temperature is 65 degrees Celsius.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate: step 1/1.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site16-19NADP+ (UniProtKB | ChEBI)
Binding site40-42NADP+ (UniProtKB | ChEBI)
Binding site75-78NADP+ (UniProtKB | ChEBI)
Binding site98-100NADP+ (UniProtKB | ChEBI)
Binding site100substrate
Binding site127-131NADP+ (UniProtKB | ChEBI)
Binding site130substrate
Binding site154substrate
Binding site160-161substrate
Binding site179substrate
Binding site205substrate
Binding site255substrate
Binding site284substrate

GO annotations

AspectTerm
Molecular Functiondiaminopimelate dehydrogenase activity
Biological Processdiaminopimelate biosynthetic process
Biological Processlysine biosynthetic process via diaminopimelate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Meso-diaminopimelate D-dehydrogenase
  • EC number
  • Short names
    DAPDH; Meso-DAP dehydrogenase

Gene names

    • Name
      ddh
    • Ordered locus names
      Cthe_0922

Organism names

Accessions

  • Primary accession
    A3DDX7

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004171231-334Meso-diaminopimelate D-dehydrogenase

Expression

Induction

Constitutively expressed.

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    334
  • Mass (Da)
    37,224
  • Last updated
    2007-03-20 v1
  • Checksum
    7A2E8D84A3DA542B
MGGVTLEKIRIGIVGYGNLGKGAELGIRQNKDMELVGIFTRRNPNSIKPLTEGVKVYSVDSARDMADKIDVMLLCSGSRTDLPVQGPEFAAMFNIVDGFDTHNKIQEYFESVDAKAKESKKVAVIACGWDPGMFSLNRLFGEVILPEGKTYTFWGKGVSQGHSDAIRRVKGVVDAKQYTIPVESAIELVRKGENPELTTRQKHIRECFVVVEEGADKERIEREIKTMPDYFADYDTIVHFISLEELKEKHSGIPHGGFSIRTGRTGINNENKHTIEYSLKLDSNPDFTANTLLAYARAAYRLNKEGVFGAKTVFDIPPAYLSPKSAEELRRSLL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000568
EMBL· GenBank· DDBJ
ABN52156.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help