A3DDX7 · DAPDH_ACET2
- ProteinMeso-diaminopimelate D-dehydrogenase
- Geneddh
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids334 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the reversible NADPH-dependent reductive amination of L-2-amino-6-oxopimelate, the acyclic form of L-tetrahydrodipicolinate, to generate the meso compound, D,L-2,6-diaminopimelate. Probably plays a role in lysine biosynthesis. Exhibits a high substrate specificity for meso-2,6-diaminopimelate (m-DAP), since the activity with L,L-2,6-diaminopimelate is less than 5% of the activity observed with m-DAP. Can use NAD+ only very poorly since the activity observed in the presence of NAD+ is about 0.3% of that with NADP+.
Catalytic activity
- meso-2,6-diaminoheptanedioate + NADP+ + H2O = (S)-2-amino-6-oxoheptanedioate + NH4+ + NADPH + H+
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.07 mM | meso-2,6-diaminoheptanedioate | 10.5 | 30 | |||
0.13 mM | NADP+ | 10.5 | 30 | |||
0.09 mM | L-2-amino-6-oxoheptanedioate | 10.5 | 30 | |||
0.21 mM | NADPH | 10.5 | 30 | |||
195 mM | ammonia | 10.5 | 30 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
15.45 μmol/min/mg | 10.5 | 30 | for the reductive amination of L-2-amino-6-oxopimelate | ||
0.24 μmol/min/mg | 10.5 | 30 | for the oxidative deamination of meso-2,6-diaminopimelate |
Temperature Dependence
Optimum temperature is 65 degrees Celsius.
Pathway
Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate: step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 16-19 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 40-42 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 75-78 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 98-100 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 100 | substrate | |||
Binding site | 127-131 | NADP+ (UniProtKB | ChEBI) | |||
Binding site | 130 | substrate | |||
Binding site | 154 | substrate | |||
Binding site | 160-161 | substrate | |||
Binding site | 179 | substrate | |||
Binding site | 205 | substrate | |||
Binding site | 255 | substrate | |||
Binding site | 284 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | diaminopimelate dehydrogenase activity | |
Biological Process | diaminopimelate biosynthetic process | |
Biological Process | lysine biosynthetic process via diaminopimelate |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMeso-diaminopimelate D-dehydrogenase
- EC number
- Short namesDAPDH; Meso-DAP dehydrogenase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Oscillospiraceae > Acetivibrio
Accessions
- Primary accessionA3DDX7
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000417123 | 1-334 | Meso-diaminopimelate D-dehydrogenase | ||
Expression
Induction
Constitutively expressed.
Interaction
Structure
Sequence
- Sequence statusComplete
- Length334
- Mass (Da)37,224
- Last updated2007-03-20 v1
- Checksum7A2E8D84A3DA542B
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000568 EMBL· GenBank· DDBJ | ABN52156.1 EMBL· GenBank· DDBJ | Genomic DNA |