A3DBR2 · A3DBR2_ACET2
- ProteintRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids434 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
function
Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.
Catalytic activity
- N6-L-threonylcarbamoyladenosine37 in tRNA + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N6-L-threonylcarbamoyladenosine37 in tRNA + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H+
Cofactor
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | metal ion binding | |
Molecular Function | N6-threonylcarbomyladenosine methylthiotransferase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Oscillospiraceae > Acetivibrio
Accessions
- Primary accessionA3DBR2
Proteomes
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-114 | MTTase N-terminal | ||||
Sequence: KRAAFYTLGCKVNQYETEAISEMFEKAGYKIVDFEDEADVYVINTCTVTNLSDRKSRQMIRRAKRNNENSIVIVIGCYAQTAPEEVSKIEGVNLVVGTKDRSRILEYLKELET | ||||||
Domain | 139-368 | Radical SAM core | ||||
Sequence: YKERTRAFIKIQEGCNQFCTYCIIPYARGPVRSRSEENILKEVSGLAHSGYKEVVLTGIHVASYGKDIKNTSLIDIIRKVHEIEGIERIRLGSIEPTTVTEEFVRAIKGMEKLCPQFHISLQSGCDSTLKRMNRKYTTKEYLRSVELLRENLKDVAVTTDVMVGFPGETDEEFNETCRFVEKVLFARMHVFKYSRRKGTPAASYPDQVAPQKKEERSRILIELASRMTLE | ||||||
Domain | 371-434 | TRAM | ||||
Sequence: KSFTGRVLPVLFEQEVKGKEGFMEGLTPNYIRVECKGDKDIEGQILNVLLREAKDDYIVGEIVN |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length434
- Mass (Da)49,691
- Last updated2007-03-20 v1
- ChecksumFB7F868991CD2383
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000568 EMBL· GenBank· DDBJ | ABN51391.1 EMBL· GenBank· DDBJ | Genomic DNA |