A3D3E8 · ACSA_SHEB5

Function

function

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site.

165050100150200250300350400450500550600650
TypeIDPosition(s)Description
Binding site191-194CoA (UniProtKB | ChEBI)
Binding site311CoA (UniProtKB | ChEBI)
Binding site335CoA (UniProtKB | ChEBI)
Binding site387-389ATP (UniProtKB | ChEBI)
Binding site411-416ATP (UniProtKB | ChEBI)
Binding site500ATP (UniProtKB | ChEBI)
Binding site515ATP (UniProtKB | ChEBI)
Binding site523CoA (UniProtKB | ChEBI)
Binding site526ATP (UniProtKB | ChEBI)
Binding site537Mg2+ (UniProtKB | ChEBI)
Binding site539Mg2+ (UniProtKB | ChEBI)
Binding site542Mg2+ (UniProtKB | ChEBI)
Binding site584CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionacetate-CoA ligase activity
Molecular FunctionAMP binding
Molecular FunctionATP binding
Molecular Functionmetal ion binding
Biological Processacetyl-CoA biosynthetic process from acetate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acetyl-coenzyme A synthetase
  • EC number
  • Short names
    AcCoA synthetase
    ; Acs
  • Alternative names
    • Acetate--CoA ligase
    • Acyl-activating enzyme

Gene names

    • Name
      acsA
    • Ordered locus names
      Sbal_1752

Organism names

Accessions

  • Primary accession
    A3D3E8

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_10000653161-650Acetyl-coenzyme A synthetase
Modified residue609N6-acetyllysine

Post-translational modification

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.

Keywords

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    650
  • Mass (Da)
    72,396
  • Last updated
    2007-03-20 v1
  • Checksum
    0A9257FD9BB53BF8
MSSQSLYKVSGNIAANALVNNDKYKTMYQESIVNPEGFWREHGKRIDWIKPYTKIKKTSFDDHNLSINWFYDGTLNASANCLDRHLAEHSDRVAIIWEGDNASEQRKITYGELHADVCKFANALRSQGVRRGDIVTIYMPMVPEAAVAMLACARIGAVHSVVFGGFSPDSIASRVIDGKSKVIITSDEGMRGGRAIPLKRNIDDALKNPDVTTVEKVIVLKRTGGKVDWVEGRDVWWHSLMETASEYCQPEEMDAEAPLFLLYTSGSTGNPKGVLHTTGGYMVYASMTHEYVFDYKAGEVYWCTADVGWITGHSYMVYGPLANGATVLIHEGVPNHPSPARLGEMIDRHKVSILYTAPTLIRALMAEGKQHFDKFDGSSLRIMGSVGEPINPEAWRWYHEVIGHEHCPIVDTWWQTETGGILITPLPGATDTKPGSATRPFFGVQPALVDNMGNILEGENEGNLVLLDSWPGQMRTVYGDHERFVLTYFKTFRGMYFTGDGARRDEDGYYWITGRVDDVINVSGHRLGTAEVESALVSHELVAEAAVVGYPHDIKGQGIYAYVTLTRGTEETEELRQELRQWVRKEIGALATPDLIQWATGLPKTRSGKIMRRFLRKIAANEVTNLGDASTLADPAVIETLIESRLNRTE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000563
EMBL· GenBank· DDBJ
ABN61261.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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