A2XN66 · CESA2_ORYSI
- ProteinProbable cellulose synthase A catalytic subunit 2 [UDP-forming]
- GeneCESA2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1073 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Probable catalytic subunit of cellulose synthase terminal complexes ('rosettes'), required for beta-1,4-glucan microfibril crystallization, a major mechanism of the cell wall formation.
Catalytic activity
- [(1->4)-beta-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->4)-beta-D-glucosyl](n+1) + H+ + UDP
[(1→4)-β-D-glucosyl](n) RHEA-COMP:10033 + CHEBI:58885 = [(1→4)-β-D-glucosyl](n+1) RHEA-COMP:10034 + CHEBI:15378 + CHEBI:58223
Cofactor
Protein has several cofactor binding sites:
Note: Binds 2 Zn2+ ions per subunit.
Pathway
Glycan metabolism; plant cellulose biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 13 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 16 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 32 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 35 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 40 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 43 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 55 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 58 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 353 | UDP-alpha-D-glucose (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 359 | UDP-alpha-D-glucose (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 360 | UDP-alpha-D-glucose (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 389 | |||||
Sequence: D | ||||||
Binding site | 389 | UDP-alpha-D-glucose (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 530 | UDP-alpha-D-glucose (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 531 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 555 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 773 | |||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | cellulose synthase (UDP-forming) activity | |
Molecular Function | metal ion binding | |
Biological Process | cell wall organization | |
Biological Process | cellulose biosynthetic process | |
Biological Process | plant-type cell wall organization or biogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable cellulose synthase A catalytic subunit 2 [UDP-forming]
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Poales > Poaceae > BOP clade > Oryzoideae > Oryzeae > Oryzinae > Oryza > Oryza sativa
Accessions
- Primary accessionA2XN66
Proteomes
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-270 | Cytoplasmic | ||||
Sequence: MDGAKSGKQCHVCQICGDGVGTAADGELFTACDVCGFPVCRPCYEYERKDGSQACPQCKTKYKRHKGSPPILGDESDDVDADDASDVNYPTSGNQDHKHKIAERMLTWRMNSGRNDDIVHSKYDSGEIGHPKYDSGEIPRIYIPSLTHSQISGEIPGASPDHMMSPVGNIGRRGHPFPYVNHSPNPSREFSGSLGNVAWKERVDGWKMKDKGAIPMANGTSIAPSEGRGVGDIDASTDYNMEDALLNDETRQPLSRKVPISSSRINPYRM | ||||||
Transmembrane | 271-291 | Helical | ||||
Sequence: VIVLRLIVLCIFLHYRITNPV | ||||||
Topological domain | 292-293 | Extracellular | ||||
Sequence: RN | ||||||
Transmembrane | 294-314 | Helical | ||||
Sequence: AYPLWLLSVICEIWFALSWIL | ||||||
Topological domain | 315-856 | Cytoplasmic | ||||
Sequence: DQFPKWSPINRETYLDRLALRYDREGEPSQLAPVDIFVSTVDPMKEPPLVTANTVLSILAVDYPVDKVSCYVSDDGAAMLTFDALAETSEFARKWVPFCKKYSIEPRAPEWYFAQKIDYLKDKVQASFVKDRRAMKREYEEFKVRVNALVAKAQKVPEEGWIMQDGTPWPGNNTRDHPGMIQVFLGHSGGLDTEGNELPRLVYVSREKRPGFQHHKKAGAMNALVRVSAVLTNGQYLLNLDCDHYINNSKALREAMCFLMDPNLGRRVCYVQFPQRFDGIDRNDRYANRNTVFFDINLRGLDGLQGPVYVGTGCVFNRTALYGYEPPIKQKRPGYFSSLCGGRKKTKKSKEKSTEKKKSHKHVDSSVPVFNLEDIEEGIEGSGFDDEKSLLMSQMSLEKRFGQSSVFVASTLMEYGGVPQSATPESLLKEAIHVISCGYEDKSDWGTEIGWIYGSVTEDILTGFKMHARGWRSIYCMPKRPAFKGSAPINLSDRLNQVLRWALGSVEILFSRHCPIWYGYGGRLKFLERFAYINTTIYPLTS | ||||||
Transmembrane | 857-877 | Helical | ||||
Sequence: IPLLLYCILPAICLLTGKFII | ||||||
Topological domain | 878-882 | Extracellular | ||||
Sequence: PEISN | ||||||
Transmembrane | 883-903 | Helical | ||||
Sequence: FASIWFISLFLSIFATGILEM | ||||||
Topological domain | 904-918 | Cytoplasmic | ||||
Sequence: RWSGVGIDEWWRNEQ | ||||||
Transmembrane | 919-939 | Helical | ||||
Sequence: FWVIGGISAHLFAVFQGLLKV | ||||||
Topological domain | 940-969 | Extracellular | ||||
Sequence: LAGIDTSFTVTSKASDEEGDFAELYMFKWT | ||||||
Transmembrane | 970-990 | Helical | ||||
Sequence: TLLIPPTTILIINLVGVVAGI | ||||||
Topological domain | 991-1001 | Cytoplasmic | ||||
Sequence: SYAINSGYQSW | ||||||
Transmembrane | 1002-1022 | Helical | ||||
Sequence: GPLFGKLFFAFWVIVHLYPFL | ||||||
Topological domain | 1023-1031 | Extracellular | ||||
Sequence: KGLMGRQNR | ||||||
Transmembrane | 1032-1052 | Helical | ||||
Sequence: TPTIVVVWAILLASIFSLLWV | ||||||
Topological domain | 1053-1073 | Cytoplasmic | ||||
Sequence: RIDPFTTRVTGPDTQKCGINC |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000319359 | 1-1073 | Probable cellulose synthase A catalytic subunit 2 [UDP-forming] | |||
Sequence: MDGAKSGKQCHVCQICGDGVGTAADGELFTACDVCGFPVCRPCYEYERKDGSQACPQCKTKYKRHKGSPPILGDESDDVDADDASDVNYPTSGNQDHKHKIAERMLTWRMNSGRNDDIVHSKYDSGEIGHPKYDSGEIPRIYIPSLTHSQISGEIPGASPDHMMSPVGNIGRRGHPFPYVNHSPNPSREFSGSLGNVAWKERVDGWKMKDKGAIPMANGTSIAPSEGRGVGDIDASTDYNMEDALLNDETRQPLSRKVPISSSRINPYRMVIVLRLIVLCIFLHYRITNPVRNAYPLWLLSVICEIWFALSWILDQFPKWSPINRETYLDRLALRYDREGEPSQLAPVDIFVSTVDPMKEPPLVTANTVLSILAVDYPVDKVSCYVSDDGAAMLTFDALAETSEFARKWVPFCKKYSIEPRAPEWYFAQKIDYLKDKVQASFVKDRRAMKREYEEFKVRVNALVAKAQKVPEEGWIMQDGTPWPGNNTRDHPGMIQVFLGHSGGLDTEGNELPRLVYVSREKRPGFQHHKKAGAMNALVRVSAVLTNGQYLLNLDCDHYINNSKALREAMCFLMDPNLGRRVCYVQFPQRFDGIDRNDRYANRNTVFFDINLRGLDGLQGPVYVGTGCVFNRTALYGYEPPIKQKRPGYFSSLCGGRKKTKKSKEKSTEKKKSHKHVDSSVPVFNLEDIEEGIEGSGFDDEKSLLMSQMSLEKRFGQSSVFVASTLMEYGGVPQSATPESLLKEAIHVISCGYEDKSDWGTEIGWIYGSVTEDILTGFKMHARGWRSIYCMPKRPAFKGSAPINLSDRLNQVLRWALGSVEILFSRHCPIWYGYGGRLKFLERFAYINTTIYPLTSIPLLLYCILPAICLLTGKFIIPEISNFASIWFISLFLSIFATGILEMRWSGVGIDEWWRNEQFWVIGGISAHLFAVFQGLLKVLAGIDTSFTVTSKASDEEGDFAELYMFKWTTLLIPPTTILIINLVGVVAGISYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRQNRTPTIVVVWAILLASIFSLLWVRIDPFTTRVTGPDTQKCGINC |
PTM databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for zinc finger, region, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Zinc finger | 13-59 | RING-type; degenerate | ||||
Sequence: CQICGDGVGTAADGELFTACDVCGFPVCRPCYEYERKDGSQACPQCK | ||||||
Region | 66-98 | Disordered | ||||
Sequence: KGSPPILGDESDDVDADDASDVNYPTSGNQDHK | ||||||
Coiled coil | 443-470 | |||||
Sequence: VKDRRAMKREYEEFKVRVNALVAKAQKV | ||||||
Region | 655-676 | Disordered | ||||
Sequence: GGRKKTKKSKEKSTEKKKSHKH |
Sequence similarities
Belongs to the glycosyltransferase 2 family. Plant cellulose synthase subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,073
- Mass (Da)120,682
- Last updated2007-03-20 v1
- Checksum55B677033E77A7BE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CM000128 EMBL· GenBank· DDBJ | EAY92276.1 EMBL· GenBank· DDBJ | Genomic DNA |