A2VEI2 · MICU1_DROME
- ProteinCalcium uptake protein 1 homolog, mitochondrial
- GeneMICU1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids525 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Calcium sensor of the mitochondrial calcium uniporter (MCU) channel, which senses calcium level via its EF-hand domains (PubMed:27568554).
At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (By similarity).
At higher calcium levels, calcium-binding to MICU1 induces a conformational change that weakens MCU-MICU1 interactions and moves MICU1 away from the pore, allowing calcium permeation through the MCU channel (By similarity).
Also required to protect against manganese toxicity by preventing manganese uptake by MCU (By similarity).
During development, required in alpha/beta or gamma mushroom body neurons to support olfactory intermediate-term memory in the adult (PubMed:27568554).
At low calcium levels, MICU1 occludes the pore of the MCU channel, preventing mitochondrial calcium uptake (By similarity).
At higher calcium levels, calcium-binding to MICU1 induces a conformational change that weakens MCU-MICU1 interactions and moves MICU1 away from the pore, allowing calcium permeation through the MCU channel (By similarity).
Also required to protect against manganese toxicity by preventing manganese uptake by MCU (By similarity).
During development, required in alpha/beta or gamma mushroom body neurons to support olfactory intermediate-term memory in the adult (PubMed:27568554).
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 281 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 283 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 285 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 287 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 292 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 472 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 474 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 476 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 478 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 483 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial intermembrane space | |
Cellular Component | uniplex complex | |
Molecular Function | calcium ion binding | |
Biological Process | anesthesia-resistant memory | |
Biological Process | calcium import into the mitochondrion | |
Biological Process | medium-term memory | |
Biological Process | mitochondrial calcium ion homeostasis | |
Biological Process | positive regulation of mitochondrial calcium ion concentration |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCalcium uptake protein 1 homolog, mitochondrial
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionA2VEI2
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
RNAi-mediated knockdown results in lethality (PubMed:27568554).
RNAi-mediated knockdown in neurons shortens lifespan and severely impairs climbing ability (PubMed:28198506).
RNAi-mediated knockdown in the developing eye decreases ommatidia number and disrupts ommatidial array (PubMed:28198506).
RNAi-mediated knockdown in neurons and, more specifically, in alpha/beta or gamma mushroom body neurons, impairs mitochondrial calcium entry and decreases intermediate-term memory after conditioning (PubMed:27568554).
Does not affect olfactory learning (PubMed:27568554).
RNAi-mediated knockdown in neurons shortens lifespan and severely impairs climbing ability (PubMed:28198506).
RNAi-mediated knockdown in the developing eye decreases ommatidia number and disrupts ommatidial array (PubMed:28198506).
RNAi-mediated knockdown in neurons and, more specifically, in alpha/beta or gamma mushroom body neurons, impairs mitochondrial calcium entry and decreases intermediate-term memory after conditioning (PubMed:27568554).
Does not affect olfactory learning (PubMed:27568554).
PTM/Processing
Features
Showing features for chain, transit peptide.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000399812 | ?-525 | Calcium uptake protein 1 homolog, mitochondrial | |||
Sequence: MSVLRFLVTRQALAALTRPRTLNIIQNPAQIAYASTLCNQNSNHNAKDLTKSSANLSLMQTRGHKRFGHQEEKTPSVTKYFHMFILSLFLISVMDWGKVKRMLTPKVDADAGQRPSSAADVNGEDKSSESESEDSEDEEAGSDLHLHEGKKIREKVGFRERKIIEYENRIRQFSTPDKVFRYFATIQVPVADDRHEVYMTPTDFLTSMTPGMKQPDGLGLDQYRRYDPKSVGEQLNLHLEKNSIFYKLGSYGLITFSDYIFLLTVLSISRRHFEIAFRMFDLNGDGDVDCEEFEMVATLVRQQTSMGTRHRDHANTGNTFKGVNSALITYFFGPNMDEKLTIEKFLDFQEQLQREILSLEFERKEPNDEGNITEADFAELLLAYAGYPLKKKQKKLKRVKRRFRDHGKGISKQDYLDFFHFLNNINDVDTALTFYHIAGASIDQQTLQHVAKTVAMVNLSDHVVDVVFTIFDENNDNQLSNKEFISVMKNRVQRGLEKPKDTGFLKMMRSVFKCAKETKPVLLDI | ||||||
Transit peptide | 1-? | Mitochondrion |
Proteomic databases
Expression
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 109-146 | Disordered | ||||
Sequence: ADAGQRPSSAADVNGEDKSSESESEDSEDEEAGSDLHL | ||||||
Domain | 268-303 | EF-hand 1 | ||||
Sequence: ISRRHFEIAFRMFDLNGDGDVDCEEFEMVATLVRQQ | ||||||
Domain | 459-494 | EF-hand 2 | ||||
Sequence: LSDHVVDVVFTIFDENNDNQLSNKEFISVMKNRVQR |
Domain
The EF-hand domains have high affinity for calcium and act as sensors of calcium levels.
Sequence similarities
Belongs to the MICU1 family. MICU1 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
A2VEI2-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- NameB
- Length525
- Mass (Da)60,344
- Last updated2007-03-20 v1
- ChecksumEB00098E8C9C1D23
A2VEI2-2
- NameA
- Differences from canonical
- 179-212: VFRYFATIQVPVADDRHEVYMTPTDFLTSMTPGM → IFRYFATVRLQDATQTIVCMTPEDFLRSIYPGI
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_039911 | 179-212 | in isoform A | |||
Sequence: VFRYFATIQVPVADDRHEVYMTPTDFLTSMTPGM → IFRYFATVRLQDATQTIVCMTPEDFLRSIYPGI |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE014134 EMBL· GenBank· DDBJ | AAF52489.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE014134 EMBL· GenBank· DDBJ | ABV53641.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY051995 EMBL· GenBank· DDBJ | AAK93419.1 EMBL· GenBank· DDBJ | mRNA | ||
BT030151 EMBL· GenBank· DDBJ | ABN49290.1 EMBL· GenBank· DDBJ | mRNA |