A2SHH0 · TYSY_METPP

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.

Catalytic activity

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

128320406080100120140160180200220240260280
TypeIDPosition(s)Description
Binding site33dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site63(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site138-139dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Active site158Nucleophile
Binding site185-188dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site188(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)
Binding site196dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site226-228dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site282(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionthymidylate synthase activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Thymidylate synthase
  • EC number
  • Short names
    TS
    ; TSase

Gene names

    • Name
      thyA
    • Ordered locus names
      Mpe_A2051

Organism names

Accessions

  • Primary accession
    A2SHH0

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003214711-283Thymidylate synthase

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    283
  • Mass (Da)
    31,957
  • Last updated
    2007-03-06 v1
  • Checksum
    336F46A78B742935
MSALPSPSPRPARSQYEDFLRHVRDHGVVKADRTGTGTTSVFGHQMRFDLREGFPLVTTKKVHLKSIVLELLWFLRGDGNATWLQERGVTIWDEWAAPDGDLGPVYGVQWRSWPTPDGGHVDQIAEVVKQLKTNPDSRRIIVSAWNVADLPKMALMPCHAFFQFYVAPGDSPSARGRLSCQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLEVGDFVWTGGDCHIYSNHREQVELQLSRAPRPYPTLQIKRRPPSIFDYAYEDFEIIGYDPHPAIKAPVAV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000555
EMBL· GenBank· DDBJ
ABM95009.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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