A2SGN1 · A2SGN1_METPP
- ProteinPhosphoribosylformylglycinamidine synthase
- GenepurL
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1336 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Catalytic activity
- ATP + H2O + L-glutamine + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N1-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H+ + L-glutamate + phosphate
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 319-330 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GASTGAGGEIRD | ||||||
Binding site | 695 | ATP (UniProtKB | ChEBI) | ||||
Sequence: A | ||||||
Binding site | 696 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 735 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 739 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 902 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 904 | ATP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 1180 | Nucleophile | ||||
Sequence: C | ||||||
Active site | 1301 | |||||
Sequence: H | ||||||
Active site | 1303 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoribosylformylglycinamidine synthase activity | |
Biological Process | 'de novo' IMP biosynthetic process | |
Biological Process | glutamine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended namePhosphoribosylformylglycinamidine synthase
- EC number
- Short namesFGAM synthase ; FGAMS
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Sphaerotilaceae > Methylibium
Accessions
- Primary accessionA2SGN1
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 41-160 | Phosphoribosylformylglycinamidine synthase N-terminal | ||||
Sequence: HVHWVAAEAALDAAARDKLAGLLRYGNAYAGPTEGTLVLVMPRLGTVSPWASKATDIAHNCGLGIRRVERVTEYRLTLKGGLLGGSKPLTDAERAAVAELLHDRMTESVAFEHGAAAHLF | ||||||
Domain | 181-230 | Phosphoribosylformylglycinamidine synthase linker | ||||
Sequence: LEAANRDFGLALSDDEIDYLVAAFTGLQRNPTDVELMMFAQANSEHCRHK | ||||||
Domain | 445-596 | PurM-like C-terminal | ||||
Sequence: AGTLLIQLGGPGMRIGMGGGAASSMAAGANTAALDFDSVQRGNPEIQRRAQEVLNHCWALGAGNPILAVHDVGAGGISNAFPELVHDAGRGARFDLRKVPLEESGLAPKEIWCNESQERYVLAIAPESLPLFEAMATRERCPYAVVGVASDA | ||||||
Domain | 845-1012 | PurM-like C-terminal | ||||
Sequence: ILIDLGQGRNRLGGSILAQTLEQLGDSAPDLDDAALLKQLVAAIHRLRGEGRLLAYHDRSDGGLWATVCEMAFAGHCGVSLNVDLLVTEGDGVSDSRMDSGDAKNWAGQVGARREELTLKALFAEELGAVIQVAGAERDKVLHVLREAGLGRHSHVIGKPNDRGVVEV |
Sequence similarities
In the N-terminal section; belongs to the FGAMS family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,336
- Mass (Da)142,937
- Last updated2007-03-06 v1
- ChecksumE302F7EF41C8EA6F
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000555 EMBL· GenBank· DDBJ | ABM94720.1 EMBL· GenBank· DDBJ | Genomic DNA |