A2RSX7 · TYW5_MOUSE

  • Protein
    tRNA wybutosine-synthesizing protein 5
  • Gene
    Tyw5
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    3/5

Function

function

tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Pathway

tRNA modification; wybutosine-tRNA(Phe) biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site1062-oxoglutarate (UniProtKB | ChEBI)
Binding site160Fe cation (UniProtKB | ChEBI); catalytic
Binding site162Fe cation (UniProtKB | ChEBI); catalytic
Binding site1662-oxoglutarate (UniProtKB | ChEBI)
Binding site1752-oxoglutarate (UniProtKB | ChEBI)
Binding site235Fe cation (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Molecular Function2-oxoglutarate-dependent dioxygenase activity
Molecular Functioniron ion binding
Molecular Functionprotein homodimerization activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine37-C2)-hydroxylase activity
Biological Processwybutosine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA wybutosine-synthesizing protein 5
  • EC number
  • Alternative names
    • tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-C(2))-hydroxylase

Gene names

    • Name
      Tyw5

Organism names

  • Taxonomic identifier
  • Strain
    • C57BL/6J
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    A2RSX7
  • Secondary accessions
    • Q3UT82
    • Q4KL17

Proteomes

Organism-specific databases

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003092751-315tRNA wybutosine-synthesizing protein 5

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain102-267JmjC

Sequence similarities

Belongs to the TYW5 family.

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

A2RSX7-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    315
  • Mass (Da)
    36,633
  • Last updated
    2007-11-13 v2
  • Checksum
    26B51EF4662C55A3
MAEQRLPVPRLRGVSREQFMEHLYPQRKPLVLEGLDLGSCTSKWTVDYLSQVGGTKEVKIHVAAVPQMDFISKNFVYRTLPFNKLVQRAAEETHKEFFISEDEKYYLRSLGEDPRKDVADIRQQFPSLGGDITFPMFFREEQFFSSVFRISSPGLQLWTHYDVMDNFLIQVTGKKRITLFNPRDAQYLYLSGSKSEVLNIDSPDLDKYPLFPKARRYECSLEAGDVLFIPALWFHNVVSEEFGVGVNIFWKHLPSECYDTTDTYGNKDPVAASRAVQILDRALKTLAELPEEYRDFYARQMVLRIQDKAYSKNFE

A2RSX7-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 72-121: SKNFVYRTLPFNKLVQRAAEETHKEFFISEDEKYYLRSLGEDPRKDVADI → KLYLLTSWSREQPKKHIKNSSFQRMRNTTYGHLEKTQGRMLQTSDSSSHH
    • 122-315: Missing

Computationally mapped potential isoform sequences

There are 2 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
G3UW53G3UW53_MOUSETyw5255
E0CZH0E0CZH0_MOUSETyw5131

Features

Showing features for sequence conflict, alternative sequence.

TypeIDPosition(s)Description
Sequence conflict5in Ref. 2; AAI32290
Alternative sequenceVSP_02910772-121in isoform 2
Alternative sequenceVSP_029108122-315in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK139666
EMBL· GenBank· DDBJ
BAE24098.1
EMBL· GenBank· DDBJ
mRNA
BC099495
EMBL· GenBank· DDBJ
-mRNA No translation available.
BC132289
EMBL· GenBank· DDBJ
AAI32290.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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