A2BGM5 · FOXN4_DANRE
- ProteinForkhead box protein N4
- Genefoxn4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids550 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Transcription factor essential for neural and some non-neural tissues development. Binds to an 11-bp consensus sequence containing the invariant tetranucleotide 5'-ACGC-3'. During development of the central nervous system, required to specify the amacrine and horizontal cell fates from multipotent retinal progenitors while suppressing the alternative photoreceptor cell fates. Drives commitment of p2 progenitors to the V2b interneuron fates during spinal cord neurogenesis. In development of non-neural tissues, plays an essential role in the specification of the atrioventricular canal.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 231-327 | Fork-head | ||||
Sequence: KPIYSYSCLIAMALKNSKTGSLPVSEIYSFMKEHFPYFKTAPDGWKNSVRHNLSLNKCFEKVENKMSGSSRKGCLWALNPAKIDKMEEEMQKWKRKD |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | chromatin binding | |
Molecular Function | cis-regulatory region sequence-specific DNA binding | |
Molecular Function | DNA-binding transcription factor activity | |
Molecular Function | DNA-binding transcription factor activity, RNA polymerase II-specific | |
Molecular Function | sequence-specific DNA binding | |
Biological Process | amacrine cell differentiation | |
Biological Process | atrioventricular canal development | |
Biological Process | heart looping | |
Biological Process | heart valve development | |
Biological Process | positive regulation of DNA-templated transcription | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | regulation of heart contraction | |
Biological Process | retina layer formation | |
Biological Process | ventral spinal cord interneuron fate commitment |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameForkhead box protein N4
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Ostariophysi > Cypriniformes > Danionidae > Danioninae > Danio
Accessions
- Primary accessionA2BGM5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
At 36-48 hpf, mutants exhibit pericardial edema due to dysmorphic hearts that fail to loop and form and atrioventricular (AV) canal. Hearts show continues peristatic pumping rather than coordinated and sequential beating of atrial and ventricular chambers. Endocardial cells remain squamous and AV myocardial cell shape fail to occur.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000428907 | 1-550 | Forkhead box protein N4 | |||
Sequence: MTVQSKLQGRGKFKKRFFRAGQQVPRPTLELSSVWLSKIFYNPEQHHNKQKMIESGITTRMSGIHENPGQSHHTSAQDYRLLTTDPSQLKDELPGDLQSLSWLTSVDVPRLQQIGGGRPDFTSSAQSSLLERQTAQLNSMTVAGGAGSAIHLQSEMQHSPLAINSMPQFSPGFPCAASVYQTAPQQVLTFTQANQQCSPGGLYGNYNSQNLFPQPRITAHSQDLQPKCFPKPIYSYSCLIAMALKNSKTGSLPVSEIYSFMKEHFPYFKTAPDGWKNSVRHNLSLNKCFEKVENKMSGSSRKGCLWALNPAKIDKMEEEMQKWKRKDLPAIRRSMANPDELDKLITDRPESCRQKSVDPGMTRLPSCPPGQTLPLAAQMQPQPVVTLSLQCLPMHQHLQLQLQNQSRLAPSSPAPAQTPPLHTVPDMTNSSLPQHPAKQHTDFYTVHTDVNSEVDALDPSIMDFAWQGNLWEEMKDDSFNLEALGTLSNSPLRLSDCDLDTSSVTPVSSAGGLPYPDLQVTGLYSSYSAIDALSNQYMNTQGGTKPIVLL |
Proteomic databases
Expression
Tissue specificity
Isoform 1 is expressed mainly in adult thymus. Isoform 2 is detected in adult skin. Isoform 3 is expressed in adult brain and embryo. Prominent expression sites include the olfactory placode, the basal layer of the olfactory epithelium, the neuroepithelium of the developing retina, the germinal zone of the differentiated eye, regions of motoneuron development in the neural tube and periventricular regions of the brain.
Developmental stage
In embryo, expressed ubiquitously, but with decreasing intensity, from the one-cell through gastrula stages.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 402-437 | Disordered | ||||
Sequence: LQNQSRLAPSSPAPAQTPPLHTVPDMTNSSLPQHPA | ||||||
Compositional bias | 422-437 | Polar residues | ||||
Sequence: HTVPDMTNSSLPQHPA |
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative promoter usage.
A2BGM5-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length550
- Mass (Da)60,968
- Last updated2007-02-20 v1
- ChecksumD030E6C2022B33C0
A2BGM5-2
- Name2
- Differences from canonical
- 1-134: MTVQSKLQGRGKFKKRFFRAGQQVPRPTLELSSVWLSKIFYNPEQHHNKQKMIESGITTRMSGIHENPGQSHHTSAQDYRLLTTDPSQLKDELPGDLQSLSWLTSVDVPRLQQIGGGRPDFTSSAQSSLLERQT → MAP
A2BGM5-3
- Name3
- Differences from canonical
- 1-139: Missing
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8M2BC99 | A0A8M2BC99_DANRE | foxn4 | 549 |
Features
Showing features for alternative sequence, sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_054327 | 1-134 | in isoform 2 | |||
Sequence: MTVQSKLQGRGKFKKRFFRAGQQVPRPTLELSSVWLSKIFYNPEQHHNKQKMIESGITTRMSGIHENPGQSHHTSAQDYRLLTTDPSQLKDELPGDLQSLSWLTSVDVPRLQQIGGGRPDFTSSAQSSLLERQT → MAP | ||||||
Alternative sequence | VSP_054326 | 1-139 | in isoform 3 | |||
Sequence: Missing | ||||||
Sequence conflict | 8 | in Ref. 1; CAB64948, 2; AAG27086 and 4; AAI28812 | ||||
Sequence: Q → H | ||||||
Sequence conflict | 39 | in Ref. 1; CAB64948 | ||||
Sequence: I → V | ||||||
Compositional bias | 422-437 | Polar residues | ||||
Sequence: HTVPDMTNSSLPQHPA |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AJ252024 EMBL· GenBank· DDBJ | CAB64948.1 EMBL· GenBank· DDBJ | mRNA | ||
AF198446 EMBL· GenBank· DDBJ | AAG27086.2 EMBL· GenBank· DDBJ | mRNA | ||
AF424786 EMBL· GenBank· DDBJ | AAL17865.1 EMBL· GenBank· DDBJ | mRNA | ||
AF443870 EMBL· GenBank· DDBJ | AAL38028.1 EMBL· GenBank· DDBJ | mRNA | ||
BX510366 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BX510917 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC128811 EMBL· GenBank· DDBJ | AAI28812.1 EMBL· GenBank· DDBJ | mRNA |