A1Z7K9 · PAN2_DROME
- ProteinPAN2-PAN3 deadenylation complex catalytic subunit PAN2
- GenePAN2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1241 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1.
Catalytic activity
Cofactor
Note: Binds 2 metal cations per subunit in the catalytic exonuclease domain.
Activity regulation
Positively regulated by the regulatory subunit PAN3.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 1039 | a divalent metal cation (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 1041 | a divalent metal cation (UniProtKB | ChEBI); catalytic | ||||
Sequence: E | ||||||
Binding site | 1148 | a divalent metal cation (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 1200 | a divalent metal cation (UniProtKB | ChEBI); catalytic | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | P-body | |
Cellular Component | PAN complex | |
Molecular Function | metal ion binding | |
Molecular Function | mRNA regulatory element binding translation repressor activity | |
Molecular Function | poly(A)-specific ribonuclease activity | |
Biological Process | mRNA processing | |
Biological Process | nuclear-transcribed mRNA poly(A) tail shortening | |
Biological Process | positive regulation of cytoplasmic mRNA processing body assembly |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePAN2-PAN3 deadenylation complex catalytic subunit PAN2
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Arthropoda > Hexapoda > Insecta > Pterygota > Neoptera > Endopterygota > Diptera > Brachycera > Muscomorpha > Ephydroidea > Drosophilidae > Drosophila > Sophophora
Accessions
- Primary accessionA1Z7K9
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Shuttles between nucleus and cytoplasm.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000441673 | 1-1241 | PAN2-PAN3 deadenylation complex catalytic subunit PAN2 | |||
Sequence: MDYVYCGTDPIGASEDILSVYDAGSAPGNGHFSPSFNGFNIGTTDPEYVELVPVLADGGEHFGVSSVAFDDYEELLWMGNQGGHVTSYYTNSMQKYTSFQVHATDIVRDISTLDSGVLALTQTSLRHQIRRGLPKFTFKSNNMKEMVSMLQLSPHRLVMAGLQDELIDFDLRTLKETRIEHVGAGGCTVLRKNSRYLFAGDQLGTVTLRDLNSLSVQHTIKTHTNILSDFSVQGNLLISCGYSGRQNNLAIDRFLMVYDLRMLRLIAPIQVMIDPQMLKFLPSLTSQLAVVSSYGQVQLVDTVELSEPRVSMYQINTNGSQCLSFDISSSSQAMAFGDQSGHINMIAAVQTPQPQFNLYSRSTEFADVVPQLPMVSITDTNFPLSSVMLPHLTTGTQWFSDWPEELLRYRYHRPKTIDPEVLSNMKMQGPIGYSPNPRTARRNQIPYVIEQGGVCSPNGNGTAAATKAENGVKIIPRRYRKVELKYTKLGTQDFDFDQHNQTCFAGLEATLPNSYCNAMLQILYFTDALRVKLLEHSCIKEFCLSCELGFLFNMLDKSTASSPCQASNFLRSFRTVPEASALGLILTDRSSNVNLISLIQNWNRFILHQMHYEIFDSSKNASTYSGSVQTSTNAENAGSSETSGSSDLYDSISDENSKEDDRERSKINAETDISKIFGTKQICINRCIKCQEEKIKESILLACNLSYPNHIKDSDQYFNFGTILKRSLSSEKSIQAFCERCKKFSPTNQSVKVTSLPQILSINCGLNNEKDITFLKRQLNRCSEKTTVDAAASLSTSKPCRYGANCSRSDCHFMHPDRKSPSHTSQPNAVNNSPNGRQKSWFPLTFTMGINDQGEVQVQTQSDASSGKSEQEEETEKPPTKGLDNNRMYALHAVVCQVDDGTQKNLVSLINVQRPYHTMKLAESADDPQSQWYIFNDFSISPVSPQESVWFTLDWKVPCILFYRHVEDDSESASTTSSTVTESEETIPSESSSGSPTNLSNPFLEEIVSPMLGNLSADATLQPLQSDEMPQSGDLVAMDAEFVTLNPEENEIRPDGKTATIKPCHMSVARISCIRGQGPAEGVPFMDDYISTQEKVVDYLTQFSGIKPGDLDANFSKKRLTALKYSYQKLKYLVDVGVIFVGHGLKNDFRVINIYVPSEQIIDTVHLFHMPHHRMVSLRFLAWHFLGTKIQSETHDSIEDARTTLQLYKHYLKLQEEKKFANALKNLYERGKQLQWKVPED |
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex (By similarity) (PubMed:23932717).
Interacts with Gyf (PubMed:31114929).
Interacts with Gyf (PubMed:31114929).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | A1Z7K9 | gw Q8SY33 | 2 | EBI-193297, EBI-160693 | |
BINARY | A1Z7K9 | PAN3 Q95RR8 | 3 | EBI-193297, EBI-119468 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 359-473 | Linker | ||||
Sequence: YSRSTEFADVVPQLPMVSITDTNFPLSSVMLPHLTTGTQWFSDWPEELLRYRYHRPKTIDPEVLSNMKMQGPIGYSPNPRTARRNQIPYVIEQGGVCSPNGNGTAAATKAENGVK | ||||||
Domain | 474-966 | USP | ||||
Sequence: IIPRRYRKVELKYTKLGTQDFDFDQHNQTCFAGLEATLPNSYCNAMLQILYFTDALRVKLLEHSCIKEFCLSCELGFLFNMLDKSTASSPCQASNFLRSFRTVPEASALGLILTDRSSNVNLISLIQNWNRFILHQMHYEIFDSSKNASTYSGSVQTSTNAENAGSSETSGSSDLYDSISDENSKEDDRERSKINAETDISKIFGTKQICINRCIKCQEEKIKESILLACNLSYPNHIKDSDQYFNFGTILKRSLSSEKSIQAFCERCKKFSPTNQSVKVTSLPQILSINCGLNNEKDITFLKRQLNRCSEKTTVDAAASLSTSKPCRYGANCSRSDCHFMHPDRKSPSHTSQPNAVNNSPNGRQKSWFPLTFTMGINDQGEVQVQTQSDASSGKSEQEEETEKPPTKGLDNNRMYALHAVVCQVDDGTQKNLVSLINVQRPYHTMKLAESADDPQSQWYIFNDFSISPVSPQESVWFTLDWKVPCILFYRHV | ||||||
Compositional bias | 626-651 | Polar residues | ||||
Sequence: GSVQTSTNAENAGSSETSGSSDLYDS | ||||||
Region | 626-666 | Disordered | ||||
Sequence: GSVQTSTNAENAGSSETSGSSDLYDSISDENSKEDDRERSK | ||||||
Compositional bias | 652-666 | Basic and acidic residues | ||||
Sequence: ISDENSKEDDRERSK | ||||||
Region | 817-838 | Disordered | ||||
Sequence: DRKSPSHTSQPNAVNNSPNGRQ | ||||||
Compositional bias | 820-838 | Polar residues | ||||
Sequence: SPSHTSQPNAVNNSPNGRQ | ||||||
Region | 857-884 | Disordered | ||||
Sequence: QVQTQSDASSGKSEQEEETEKPPTKGLD | ||||||
Compositional bias | 867-881 | Basic and acidic residues | ||||
Sequence: GKSEQEEETEKPPTK | ||||||
Region | 971-1000 | Disordered | ||||
Sequence: ESASTTSSTVTESEETIPSESSSGSPTNLS | ||||||
Compositional bias | 972-1000 | Polar residues | ||||
Sequence: SASTTSSTVTESEETIPSESSSGSPTNLS | ||||||
Domain | 1036-1208 | Exonuclease | ||||
Sequence: VAMDAEFVTLNPEENEIRPDGKTATIKPCHMSVARISCIRGQGPAEGVPFMDDYISTQEKVVDYLTQFSGIKPGDLDANFSKKRLTALKYSYQKLKYLVDVGVIFVGHGLKNDFRVINIYVPSEQIIDTVHLFHMPHHRMVSLRFLAWHFLGTKIQSETHDSIEDARTTLQLY |
Domain
Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.
The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.
Sequence similarities
Belongs to the peptidase C19 family. PAN2 subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,241
- Mass (Da)138,968
- Last updated2007-02-06 v1
- ChecksumBFFE4AB585B1FB5D
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A0B4LEY4 | A0A0B4LEY4_DROME | PAN2 | 1240 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 626-651 | Polar residues | ||||
Sequence: GSVQTSTNAENAGSSETSGSSDLYDS | ||||||
Compositional bias | 652-666 | Basic and acidic residues | ||||
Sequence: ISDENSKEDDRERSK | ||||||
Compositional bias | 820-838 | Polar residues | ||||
Sequence: SPSHTSQPNAVNNSPNGRQ | ||||||
Compositional bias | 867-881 | Basic and acidic residues | ||||
Sequence: GKSEQEEETEKPPTK | ||||||
Compositional bias | 972-1000 | Polar residues | ||||
Sequence: SASTTSSTVTESEETIPSESSSGSPTNLS |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE013599 EMBL· GenBank· DDBJ | AAF59018.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT031142 EMBL· GenBank· DDBJ | ABX00764.1 EMBL· GenBank· DDBJ | mRNA |