A1Z1Z9 · A1Z1Z9_CLOPF

  • Protein
    Thiol-activated cytolysin
  • Gene
    pfoA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    2/5

Function

function

A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular region
Cellular Componenthost cell plasma membrane
Cellular Componentmembrane
Molecular Functioncholesterol binding
Molecular Functiontoxin activity
Biological Processkilling of cells of another organism

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Thiol-activated cytolysin

Gene names

    • Name
      pfoA
    • Synonyms
      pfo
    • ORF names
      G6Z34_14855

Organism names

  • Taxonomic identifier
  • Organism
  • Strains
    • AM923
    • AM937
    • NCTC 8533
    • CP-40
  • Taxonomic lineage
    Bacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Clostridium

Accessions

  • Primary accession
    A1Z1Z9

Proteomes

Subcellular Location

Membrane
; Multi-pass membrane protein
Secreted
Host cell membrane
; Multi-pass membrane protein

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-26
ChainPRO_504231194227-500Thiol-activated cytolysin

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain393-493Thiol-activated cytolysin C-terminal

Sequence similarities

Belongs to the cholesterol-dependent cytolysin family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    500
  • Mass (Da)
    55,874
  • Last updated
    2007-02-06 v1
  • Checksum
    FD3853121AF7FCB0
MIRFKKTKLIASIAMALCLFSQPVISFSKDITDKNQSIDSGISSLSYNRNEVLASNGDKIESFVPKEGKKTGNKFIVVERQKRSLTTSPVDISIIDSVNDRTYPGALQLADKAFVENRPTILMVKRKPININIDLPGLKGENSIKVDDPTYGKVSGAIDELVSKWNEKYSSTHTLPARTQYSESMVYSKSQISSALNVNAKVLENSLGVDFNAVANNEKKVMILAYKQIFYTVSADLPKNPSDLFDDSVTFNDLKQKGVSNEAPPLMVSNVAYGRTIYVKLETTSSSKDVQAAFKALIKNTDIKNSQQYKDIYENSSFTAVVLGGDAQEHNKVVTKDFDEIRKVIKDNATFSTKNPAYPISYTSVFLKDNSVAAVHNKTDYIETTSTEYSKGKINLDHSGAYVAQFEVAWDEVSYDKEGNEVLTHKTWDGNYQDKTSHYSTVIPLEANVRNIRIKARECTGLAWEWWRDVISEYDVPLTNNINVSIWGTTLYPGSSITYN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
EF165972
EMBL· GenBank· DDBJ
ABM21536.1
EMBL· GenBank· DDBJ
Genomic DNA
EF165973
EMBL· GenBank· DDBJ
ABM21537.1
EMBL· GenBank· DDBJ
Genomic DNA
EF165974
EMBL· GenBank· DDBJ
ABM21538.1
EMBL· GenBank· DDBJ
Genomic DNA
JAALLZ010000009
EMBL· GenBank· DDBJ
NGU31363.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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