A1WWE4 · MNME_HALHL
- ProteintRNA modification GTPase MnmE
- GenemnmE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids469 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
Cofactor
Note: Binds 1 potassium ion per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 38 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 95 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 134 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 240-245 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NAGKSS | ||||||
Binding site | 244 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 259-265 | GTP (UniProtKB | ChEBI) | ||||
Sequence: SAQAGTT | ||||||
Binding site | 265 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 284-287 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTAG | ||||||
Binding site | 468 | (6S)-5-formyl-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | metal ion binding | |
Biological Process | tRNA methylation | |
Biological Process | tRNA wobble uridine modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA modification GTPase MnmE
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Chromatiales > Ectothiorhodospiraceae > Halorhodospira
Accessions
- Primary accessionA1WWE4
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000345797 | 1-469 | tRNA modification GTPase MnmE | |||
Sequence: MVHTASGREVRHGLEDDDADTICAMATPSGQGGVAVVRVSGSRALEVAEEVAGPLPAPREAGLRRFRDARGETLDHGLVLVFPGPGSYTGEDVVELQGHGSPAAVTAVLEALCAAGARPAGPGEFSERAFLNGRLDLTQAEAVASLIEAETDGARRAALRALSGAFGQRVDGLADRMIDLRALIEAFLDFPEDEDVPADPPELAAEIEALGSELAEIRRRAAAGVRFGEGIRVALVGPPNAGKSSLLNVLSGEEAAIVSAQAGTTRDVVRQWAALGSRHAELLDTAGLRDAEAQDEIEAEGARRARAAASEADLLLVVIEAGKTLDEELRARIAEQAPRPVVVIVNKIDASGDEAGWEGESEVGAHVRRARVSAHTGAGIEALRRGLAALVDREAGEDAWAARHRHIEALDRAGEELEEALVVARRGGQEELVAEALRRAQTALGEITGRVSHEALLGRIFSGFCIGKE |
Interaction
Subunit
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 230-392 | TrmE-type G | ||||
Sequence: GIRVALVGPPNAGKSSLLNVLSGEEAAIVSAQAGTTRDVVRQWAALGSRHAELLDTAGLRDAEAQDEIEAEGARRARAAASEADLLLVVIEAGKTLDEELRARIAEQAPRPVVVIVNKIDASGDEAGWEGESEVGAHVRRARVSAHTGAGIEALRRGLAALVD |
Sequence similarities
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length469
- Mass (Da)49,183
- Last updated2007-02-06 v1
- Checksum558FEA211E1A5C27
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000544 EMBL· GenBank· DDBJ | ABM62006.1 EMBL· GenBank· DDBJ | Genomic DNA |