A1VCZ9 · DAPA_NITV4

Function

function

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.

Features

Showing features for site, binding site, active site.

TypeIDPosition(s)Description
Site44Part of a proton relay during catalysis
Binding site45pyruvate (UniProtKB | ChEBI)
Site107Part of a proton relay during catalysis
Active site133Proton donor/acceptor
Active site162Schiff-base intermediate with substrate
Binding site204pyruvate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4-hydroxy-tetrahydrodipicolinate synthase activity
Biological Processdiaminopimelate biosynthetic process
Biological Processlysine biosynthetic process via diaminopimelate

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-tetrahydrodipicolinate synthase
  • EC number
  • Short names
    HTPA synthase

Gene names

    • Name
      dapA
    • Ordered locus names
      Dvul_1296

Organism names

Accessions

  • Primary accession
    A1VCZ9

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003409481-2924-hydroxy-tetrahydrodipicolinate synthase

Interaction

Subunit

Homotetramer; dimer of dimers.

Structure

Family & Domains

Sequence similarities

Belongs to the DapA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    292
  • Mass (Da)
    31,903
  • Last updated
    2007-02-06 v1
  • Checksum
    A33634198EF70B7D
MQFTGAFTAIVTPFRNGRVDEERFRELIEWQIEQGINGLVPCGTTGESATLSHDEHRDVIRICVEQVKGRIPVLAGAGSNNTREAIDLTRFAKEAGADGALLITPYYNKPTQEGLYLHFKAIASEVSMPFIVYNVPSRTGTNICPETLARLNRDIPEVVGVKEATGNLIQVSEILEYCGTDFQVLSGDDFTVLPLLSVGGCGVISVTSNVVPAKMSDMCRAFKAGDLATARRLHFELSPINRAMFLETNPIPVKTALALMGRIDLEMRLPLCPLQQVNQSRLRDILAAAGIL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000527
EMBL· GenBank· DDBJ
ABM28315.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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