A1RQI6 · A1RQI6_PYRIL
- Protein7-carboxy-7-deazaguanine synthase
- GenequeE
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids216 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.
Catalytic activity
- 6-carboxy-5,6,7,8-tetrahydropterin + H+ = 7-carboxy-7-deazaguanine + NH4+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 S-adenosyl-L-methionine per subunit.
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Pathway
Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12-14 | substrate | |||
Binding site | 27 | substrate | |||
Binding site | 31 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 35 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 37-39 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 38 | [4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 40 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 73 | substrate | |||
Binding site | 75 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 120-122 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | carbon-nitrogen lyase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | S-adenosyl-L-methionine binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name7-carboxy-7-deazaguanine synthase
- EC number
- Short namesCDG synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageArchaea > Thermoproteota > Thermoprotei > Thermoproteales > Thermoproteaceae > Pyrobaculum
Accessions
- Primary accessionA1RQI6
Proteomes
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 18-216 | Radical SAM core | |||
Sequence similarities
Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length216
- Mass (Da)24,370
- Last updated2007-02-06 v1
- Checksum4BBBDF626374DF09
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000504 EMBL· GenBank· DDBJ | ABL87218.1 EMBL· GenBank· DDBJ | Genomic DNA |