A1RQI6 · A1RQI6_PYRIL

Function

function

Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

S-adenosyl-L-methionine (UniProtKB | Rhea| CHEBI:59789 )

Note: Binds 1 S-adenosyl-L-methionine per subunit.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site12-14substrate
Binding site27substrate
Binding site31[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site35[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site37-39S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site38[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site40Mg2+ (UniProtKB | ChEBI)
Binding site73substrate
Binding site75S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site120-122S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functioncarbon-nitrogen lyase activity
Molecular Functionmagnesium ion binding
Molecular FunctionS-adenosyl-L-methionine binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    7-carboxy-7-deazaguanine synthase
  • EC number
  • Short names
    CDG synthase
  • Alternative names
    • Archaeosine biosynthesis protein QueE

Gene names

    • Name
      queE
    • Ordered locus names
      Pisl_0034

Organism names

Accessions

  • Primary accession
    A1RQI6

Proteomes

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain18-216Radical SAM core

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    216
  • Mass (Da)
    24,370
  • Last updated
    2007-02-06 v1
  • Checksum
    4BBBDF626374DF09
MKFRVVEIFASLQGEGVNLGKPAVFIRLAGCPIRCRYCDTKYSWDPLGGVEIDAEEVVQRAAAYGQLGHVVITGGEPLIWRNLHELACPLRRLGTVEVETSGVYPPTPQLDACVDFYDVSPKLSNARVDALLHPHYPRSPKAWFKFVVGGEEDVEEAVRYVEKHGIPRERVFLMPLAETPEQHAEVLRRIWDAAVKHKLRVTPRLHVEAWGNVRGR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000504
EMBL· GenBank· DDBJ
ABL87218.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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