A1R8V3 · RPOC_PAEAT
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1299 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 60 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 62 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 75 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 78 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 535 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 537 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 539 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 877 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 954 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 961 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 964 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Short namesRNAP subunit beta'
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micrococcales > Micrococcaceae > Paenarthrobacter
Accessions
- Primary accessionA1R8V3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000308818 | 1-1299 | DNA-directed RNA polymerase subunit beta' | |||
Sequence: MSSESSFGLMQIGLATAEDIRGWSYGEVKKPETINYRTLKPEKDGLFCEKIFGPSRDWECYCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAPVTHIWYFKGVPSRLGYLLDLAPKDLEKVIYFAAYMITSVDTESRHAELPNLQVEHDLEKKQMVDNRDSDIATIARDLEDELARLEGEGAKAADKKKARDSADRQMANVRKRADADIERLEQVWDRFKNLKVADLEGDEGLYRELRDRYGLYFEGSMGAEAIKKRLETFDMQAEAESLRDTIQNGKGQRKTRALKRLKVVNAFLTTNNSPLGMVLDAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPEIIVNNEKRMLQEAVDSLFDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKQMALELFKPFVMKRLVDLNHAQNIKSAKRMVERYRPQVWDVLEEIITEHPVLLNRAPTLHRLGIQAFEPQLVEGKAIQLHPLVCGAFNADFDGDQMAVHLPLSPEAQAEARILMLSSNNILKPSDGRPVTLPSQDMIIGLYHLTTKRVGSAGEGRIFSSVSEAIMAYDARDLHLNSQVKIRLDDFVPYAGWEAPEGWEPGQPALVETSLGQVIFNQTLPEDYPWVEAVADKGELSRIVNDLAERYPKVVTAATLDNLKDAGFYWATRSGVTVAISDIEVPTSKPAILAGYENMAAKIQGQYDKGLIDDDERRQELIEIWNKATNEIAQAMRDSLSPMNTINRMVSSGARGNWMQVRQIAGIRGLVANPKGEIIPRPIKSSYREGLSVLEYFIATHGARKGLADTALRTANSGYLTRRLVDVSQDVIVREEDCGTERGLVTPIAVPDSNGELVLDENVENSAYARTLAVDVVDAQGNVLAAGGTDCGDVVIDQLLAAGITEVKVRSVLTCESKVGTCALCYGRSLATGKTVDIGEAVGIIAAQSIGEPGTQLTMRTFHTGGAVSAGGGDDITQGLPRIQELFEARTPKGVAPIAEAAGRITIEESERQMRLVITPDDGSEEIAYPVLRRSRLLIEDGEHVSVGQKLINGPVDPKQVLRIMGPRAAQKFLVDEVQGVYRSQGIGIHDKHVEVIVRQMLRRVTVIESGDSDLLPGELAERARFEDENRRVVSEGKAPASGRPELMGITKASLATESWLSAASFQETTRVLTQAAMEGKSDPLLGLKENVIIGKLIPAGTGLPRYTEVTVEPTEEAKASLFTGPSAFTDFSYDALGGDGAPEFHAIPLDDYDLGNDFR |
Interaction
Subunit
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length1,299
- Mass (Da)143,506
- Last updated2007-02-06 v1
- Checksum48E828D5F489D5E9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000474 EMBL· GenBank· DDBJ | ABM10268.1 EMBL· GenBank· DDBJ | Genomic DNA |