A1L3G9 · NMP1B_XENLA
- ProteinNuclear envelope integral membrane protein 1b
- Genenemp1b
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids434 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
In concert with ran, required for proper eye development (PubMed:25946333).
May be involved in the expression of early eye marker genes (PubMed:19167377).
Contributes to nuclear envelope stiffness in germ cells (By similarity).
Required for fertility (By similarity).
Essential for normal erythropoiesis (By similarity).
Required for efficient nuclear envelope opening and enucleation during the late stages of erythroblast maturation (By similarity).
May be involved in the expression of early eye marker genes (PubMed:19167377).
Contributes to nuclear envelope stiffness in germ cells (By similarity).
Required for fertility (By similarity).
Essential for normal erythropoiesis (By similarity).
Required for efficient nuclear envelope opening and enucleation during the late stages of erythroblast maturation (By similarity).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | nuclear envelope | |
Cellular Component | nuclear inner membrane | |
Biological Process | erythrocyte enucleation | |
Biological Process | erythrocyte maturation | |
Biological Process | eye development |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameNuclear envelope integral membrane protein 1b
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus
Accessions
- Primary accessionA1L3G9
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Nucleus inner membrane ; Multi-pass membrane protein
Note: Localization in the nuclear membrane is essential for its function. Colocalizes with lamins and banf1-a/b at the nuclear envelope.
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 151-171 | Helical | ||||
Sequence: PRLFFVFLCGLLLFFYGDTLS | ||||||
Transmembrane | 175-195 | Helical | ||||
Sequence: LFFYSTGITVGMLASMLILVF | ||||||
Transmembrane | 206-226 | Helical | ||||
Sequence: PFFALLLGGWSVSIYVIQLVF | ||||||
Transmembrane | 239-259 | Helical | ||||
Sequence: YLIVYLGIVGFVSFAFCYIYG | ||||||
Transmembrane | 280-300 | Helical | ||||
Sequence: LLMYVSVQIQHIAVTIVVIAF |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Morpholino knockdown results in defects in eye development, reduced expression of early eye marker genes rax, tbx3, six3 and pax6 and reduction of cell density at the neurula stage. Co-knockdown of nemp1b and ran elicits reduction of cell density and eye defects more significantly than the individual knockdown of either one.
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-29 | |||||
Sequence: MAGEVEGRGCGFSLGVLVTLLVLPLPSLC | ||||||
Chain | PRO_0000332241 | 30-434 | Nuclear envelope integral membrane protein 1b | |||
Sequence: TLSTEKELHVIKLYEGRMVRYNESRNFCYQRTYEPKWSDVWTKIQIRINSTKMIRVTQVDNEEKLKEMETFNMFDFFSSFLKEKLNDTFIYVNLYSNKTCVKVHLTDTDTYYSVALSRGFDPRLFFVFLCGLLLFFYGDTLSRSQLFFYSTGITVGMLASMLILVFMLSKLMPKKSPFFALLLGGWSVSIYVIQLVFRNLQAICSEYWQYLIVYLGIVGFVSFAFCYIYGPLENERSINILNWTLQLIGLLLMYVSVQIQHIAVTIVVIAFCTKQIEYPVQWIYILYRKIKLKRAKPGPPRLLTEEEYRKQADVETRKALEELRECCSSPDFAAWKTISRIQSPKRFADFVEGSSHLTPNEVSVHEHEYGLGGSFLEDELFGEDSDVEEEMEIEPPLYPIPRSVF |
Post-translational modification
Phosphorylated.
Expression
Developmental stage
Expressed both maternally and zygotically. At the early gastrula stage, expressed mainly in the entire animal hemisphere. During neurulation, its expression becomes restricted to the anterior neuroectoderm. At the tailbud stage, expressed in various anterior regions including the anterior central nervous system (CNS), otic vesicles, and branchial arches.
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 176-287 | A; required for its colocalization with lamins at the nuclear envelope | ||||
Sequence: FFYSTGITVGMLASMLILVFMLSKLMPKKSPFFALLLGGWSVSIYVIQLVFRNLQAICSEYWQYLIVYLGIVGFVSFAFCYIYGPLENERSINILNWTLQLIGLLLMYVSVQ | ||||||
Motif | 317-325 | Nuclear localization signal | ||||
Sequence: RKIKLKRAK | ||||||
Region | 326-395 | B; interaction with ran | ||||
Sequence: PGPPRLLTEEEYRKQADVETRKALEELRECCSSPDFAAWKTISRIQSPKRFADFVEGSSHLTPNEVSVHE | ||||||
Region | 326-434 | Interaction with banf1-a and banf1-b | ||||
Sequence: PGPPRLLTEEEYRKQADVETRKALEELRECCSSPDFAAWKTISRIQSPKRFADFVEGSSHLTPNEVSVHEHEYGLGGSFLEDELFGEDSDVEEEMEIEPPLYPIPRSVF | ||||||
Region | 368-375 | BAF-binding site (BBS); essential for interaction with banf1-a, banf1-b and ran | ||||
Sequence: SRIQSPKR |
Domain
The transmembrane domains are required and sufficient for its oligomerization.
Sequence similarities
Belongs to the NEMP family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length434
- Mass (Da)50,327
- Last updated2007-02-06 v1
- Checksum258089C9B16BA011
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AB474920 EMBL· GenBank· DDBJ | BAH24056.1 EMBL· GenBank· DDBJ | mRNA | ||
BC130092 EMBL· GenBank· DDBJ | AAI30093.1 EMBL· GenBank· DDBJ | mRNA |