A1HSE3 · A1HSE3_9FIRM
- ProteinRibosomal protein uS12 methylthiotransferase RimO
- GenerimO
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids441 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein uS12.
Catalytic activity
- L-aspartate89-[ribosomal protein uS12]-hydrogen + [sulfur carrier]-SH + AH2 + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate89-[ribosomal protein uS12]-hydrogen + [sulfur carrier]-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H+
RHEA-COMP:10461 CHEBI:29961 Position: 89CHEBI:64428 + RHEA-COMP:14737 + CHEBI:17499 + 2 CHEBI:59789 = RHEA-COMP:10460 + RHEA-COMP:14739 + CHEBI:17319 + CHEBI:57844 + CHEBI:13193 + CHEBI:57856 + 2 CHEBI:15378
Cofactor
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 11 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 47 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 81 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI) | |||
Binding site | 155 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 159 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 162 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | aspartic acid methylthiotransferase activity | |
Molecular Function | metal ion binding | |
Molecular Function | protein methylthiotransferase activity | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibosomal protein uS12 methylthiotransferase RimO
- EC number
- Short namesuS12 MTTase ; uS12 methylthiotransferase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Negativicutes > Selenomonadales > Sporomusaceae > Thermosinus
Accessions
- Primary accessionA1HSE3
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-118 | MTTase N-terminal | |||
Domain | 141-371 | Radical SAM core | |||
Domain | 374-441 | TRAM | |||
Sequence similarities
Belongs to the methylthiotransferase family. RimO subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length441
- Mass (Da)49,318
- Last updated2007-02-06 v1
- ChecksumE74B991EDDB1139D
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AAWL01000015 EMBL· GenBank· DDBJ | EAX47103.1 EMBL· GenBank· DDBJ | Genomic DNA |